Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2000-9-13
pubmed:databankReference
pubmed:abstractText
Pregnancy specific glycoproteins (PSG) are secreted into the maternal circulation and may function to regulate the immune system to ensure survival of the fetal allograft. In this study, we have cloned and determined by in situ hybridization the placental sites of expression of Psg18, a murine member of the PSG family that belongs to the Ig superfamily. Recombinant PSG18 and a truncated form containing only the N-terminal domain (PSG18N) were used to treat peritoneal elicited macrophages and RAW 264.7 cells. PSG18 and PSG18N induced IL-10 mRNA expression in the presence and absence of lipopolysaccharide (LPS). IL-10 protein was also detected in the supernatant of macrophages and RAW 264.7 cells following PSG18N treatment, albeit higher concentrations were required in the absence of LPS. In contrast, treatment of these cells with PSG18N resulted in no change in the expression of IL-1/beta, TNF-alpha, inducible NO synthase, IL-12p40 and TGF-beta mRNA. Taken together, these results suggest that PSG18 selectively up-regulates IL-10 production by macrophages, providing a possible mechanism by which this protein helps promote successful pregnancy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1830-40
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10940872-Amino Acid Sequence, pubmed-meshheading:10940872-Animals, pubmed-meshheading:10940872-Base Sequence, pubmed-meshheading:10940872-Binding Sites, pubmed-meshheading:10940872-Cell Line, pubmed-meshheading:10940872-Cells, Cultured, pubmed-meshheading:10940872-Cloning, Molecular, pubmed-meshheading:10940872-Cytokines, pubmed-meshheading:10940872-DNA, Complementary, pubmed-meshheading:10940872-Female, pubmed-meshheading:10940872-Gene Expression, pubmed-meshheading:10940872-Interleukin-10, pubmed-meshheading:10940872-Kinetics, pubmed-meshheading:10940872-Macrophages, Peritoneal, pubmed-meshheading:10940872-Male, pubmed-meshheading:10940872-Mice, pubmed-meshheading:10940872-Mice, Inbred BALB C, pubmed-meshheading:10940872-Mice, Inbred C3H, pubmed-meshheading:10940872-Molecular Sequence Data, pubmed-meshheading:10940872-Placenta, pubmed-meshheading:10940872-Pregnancy, pubmed-meshheading:10940872-Pregnancy Proteins, pubmed-meshheading:10940872-Protein Structure, Tertiary, pubmed-meshheading:10940872-RNA, Messenger, pubmed-meshheading:10940872-Sequence Homology, Amino Acid, pubmed-meshheading:10940872-Spodoptera, pubmed-meshheading:10940872-Up-Regulation
pubmed:year
2000
pubmed:articleTitle
Pregnancy specific glycoprotein 18 induces IL-10 expression in murine macrophages.
pubmed:affiliation
Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda 20814, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.