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rdf:type |
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lifeskim:mentions |
umls-concept:C0001830,
umls-concept:C0006304,
umls-concept:C0012854,
umls-concept:C0029073,
umls-concept:C0042765,
umls-concept:C0178719,
umls-concept:C1334043,
umls-concept:C1514873,
umls-concept:C1546857,
umls-concept:C1547239,
umls-concept:C1556066,
umls-concept:C1619636,
umls-concept:C2911648
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pubmed:issue |
17
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pubmed:dateCreated |
2000-9-21
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pubmed:databankReference |
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pubmed:abstractText |
As part of a Brucella abortus 2308 genome project carried out in our laboratory, we identified, cloned, and sequenced a genomic DNA fragment containing a locus (virB) highly homologous to bacterial type IV secretion systems. The B. abortus virB locus is a collinear arrangement of 13 open reading frames (ORFs). Between virB1 and virB2 and downstream of ORF12, two degenerated, palindromic repeat sequences characteristic of Brucella intergenic regions were found. Gene reporter studies demonstrated that the B. abortus virB locus constitutes an operon transcribed from virB1 which is turned on during the stationary phase of growth. A B. abortus polar virB1 mutant failed to replicate in HeLa cells, indicating that the virB operon plays a critical role in intracellular multiplication. Mutants with polar and nonpolar mutations introduced in virB10 showed different behaviors in mice and in the HeLa cell infection assay, suggesting that virB10 per se is necessary for the correct function of this type IV secretion apparatus. Mouse infection assays demonstrated that the virB operon constitutes a major determinant of B. abortus virulence. It is suggested that putative effector molecules secreted by this type IV secretion system determine routing of B. abortus to an endoplasmic reticulum-related replication compartment.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10334982,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10481178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10510235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10564509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10564519,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-10580277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-2231712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-2370849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-2857162,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-3017694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-6270337,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-7557413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-7622254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-7891554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-7891556,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-7891565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-8464913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-8529885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9150199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9393796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9465074,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9484895,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9673273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9701808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9721274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10940027-9826346
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
182
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4849-55
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10940027-Agrobacterium tumefaciens,
pubmed-meshheading:10940027-Animals,
pubmed-meshheading:10940027-Bacterial Proteins,
pubmed-meshheading:10940027-Base Sequence,
pubmed-meshheading:10940027-Brucella abortus,
pubmed-meshheading:10940027-DNA, Bacterial,
pubmed-meshheading:10940027-DNA-Binding Proteins,
pubmed-meshheading:10940027-Female,
pubmed-meshheading:10940027-Genes, Bacterial,
pubmed-meshheading:10940027-HeLa Cells,
pubmed-meshheading:10940027-Humans,
pubmed-meshheading:10940027-Intracellular Fluid,
pubmed-meshheading:10940027-Mice,
pubmed-meshheading:10940027-Mice, Inbred BALB C,
pubmed-meshheading:10940027-Molecular Sequence Data,
pubmed-meshheading:10940027-Operon,
pubmed-meshheading:10940027-Recombination, Genetic,
pubmed-meshheading:10940027-Virulence,
pubmed-meshheading:10940027-Virulence Factors
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pubmed:year |
2000
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pubmed:articleTitle |
A homologue of an operon required for DNA transfer in Agrobacterium is required in Brucella abortus for virulence and intracellular multiplication.
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pubmed:affiliation |
Instituto de Investigaciones Biotecnológicas, Universidad Nacional de General San Martín, CONICET, San Martín 1650, Argentina.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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