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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-11-15
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pubmed:abstractText |
Microdissection and differential display PCR were used to identify genes preferentially expressed in the highly proliferative terminal end buds (TEBs) in the mammary gland of 45-day-old virgin rats. One clone exhibited 87% homology to the human p190-B gene encoding a novel Rho-Gap. Using in situ hybridization, p190-B was detected in both the TEBs and the terminal ducts, with the highest expression observed in the outer layer of TEBs. During normal mammary gland development, p190-B mRNA expression was highest in the virgin mammary gland and decreased during late pregnancy and lactation. Interestingly, increased levels of p190-B mRNA relative to the normal mammary gland were seen in a subset of murine mammary tumors that appeared to be less well differentiated and potentially more aggressive. Transient transfection of a p190-B expression construct into MCF-10A human mammary epithelial cells resulted in disruption of the actin cytoskeleton, which suggests a role for p190-B in regulating the signaling pathways that influence cell migration and invasion. These results suggest that p190-B may be required for virgin mammary gland development, and its aberrant expression may occur in breast cancer.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ARHGAP35 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ARHGAP5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Actins,
http://linkedlifedata.com/resource/pubmed/chemical/Arhgap5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GTPase-Activating Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Grlf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Grlf1 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1044-9523
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
343-54
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pubmed:dateRevised |
2011-9-27
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pubmed:meshHeading |
pubmed-meshheading:10939588-Actins,
pubmed-meshheading:10939588-Animals,
pubmed-meshheading:10939588-Biological Markers,
pubmed-meshheading:10939588-Breast,
pubmed-meshheading:10939588-Cell Line,
pubmed-meshheading:10939588-Coloring Agents,
pubmed-meshheading:10939588-Cytoskeleton,
pubmed-meshheading:10939588-DNA, Complementary,
pubmed-meshheading:10939588-DNA-Binding Proteins,
pubmed-meshheading:10939588-Epithelial Cells,
pubmed-meshheading:10939588-Female,
pubmed-meshheading:10939588-GTPase-Activating Proteins,
pubmed-meshheading:10939588-Gene Expression Profiling,
pubmed-meshheading:10939588-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:10939588-Humans,
pubmed-meshheading:10939588-In Situ Hybridization,
pubmed-meshheading:10939588-Mammary Glands, Animal,
pubmed-meshheading:10939588-Mammary Neoplasms, Experimental,
pubmed-meshheading:10939588-Mice,
pubmed-meshheading:10939588-Microscopy, Fluorescence,
pubmed-meshheading:10939588-Nuclear Proteins,
pubmed-meshheading:10939588-Phosphoproteins,
pubmed-meshheading:10939588-Pregnancy,
pubmed-meshheading:10939588-RNA,
pubmed-meshheading:10939588-Rats,
pubmed-meshheading:10939588-Rats, Inbred WF,
pubmed-meshheading:10939588-Recombinant Proteins,
pubmed-meshheading:10939588-Repressor Proteins,
pubmed-meshheading:10939588-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
P190-B, a Rho-GTPase-activating protein, is differentially expressed in terminal end buds and breast cancer.
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pubmed:affiliation |
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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