Linked Life Data

10938598

Source:http://linkedlifedata.com/resource/pubmed/id/10938598

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-11-24
pubmed:abstractText
To identify apoptosis of nonparenchymal cells in fibrotic livers, we established a triple staining method which combined immunohistochemistry for cell markers and Masson staining for collagen as well as terminal deoxynucleotidyl transferase UTP nick end labeling (TUNEL). Five microm formalin fixed, paraffin-embedded liver sections were prepared for staining. Firstly, TUNEL staining was carried out to detect apoptosis of liver cells. Then, the sections were subjected to immunohistochemistry for alpha-smooth muscle actin (alpha -SMA) or KP-1 to identify hepatic stellate cells or Kupffer cells. Finally, Masson staining was performed to show the relationship between apoptosis and collagens. In addition, we optimized different conditions of fixation, digestion and color development which may affect the results.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1345-4676
pubmed:author
pubmed:issnType
Print
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
280-3
pubmed:dateRevised
2011-8-1
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Triple-staining to identify apoptosis of hepatic cells in situ.
pubmed:affiliation
China-Japan Friendship Hospital, Beijing, PR China.
pubmed:publicationType
Journal Article