Linked Life Data

10938354

Source:http://linkedlifedata.com/resource/pubmed/id/10938354

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-10-18
pubmed:abstractText
Transgenic poplars (Populus tremula x Populus alba) were obtained by introduction of a sense homologous transgene encoding caffeic acid O-methyltransferase (COMT) under the control either of the cauliflower mosaic virus double 35S promoter or of the eucalyptus cinnamyl alcohol dehydrogenase promoter. Although these constructs conferred a moderate overexpression of COMT in some lines, a transgenic line with the double 35S promoter was found where COMT activity in woody tissues was close to zero due to a gene-silencing phenomenon. For the first time in COMT down-regulated trees, this alteration substantially reduced lignin level in 6-month-old trees (17% decrease). Lignin structure was found to be strongly altered, with a two times higher content in condensed bonds, an almost complete lack of syringyl units, and the incorporation of 5-hydroxyguaiacyl units to the most remarkable extent reported so far. Consistent with the higher cellulose content and with the higher condensation degree of the lignin, the impact of the transformation on the kraft-pulping performances of the poplar trees positively affected the pulp yield (10% relative increase), but made lignins less amenable to industrial degradations.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-10429249, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-10430877, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-10468559, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-10535921, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-16668718, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-1932694, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-2408008, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-6326095, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-7610157, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-7727744, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-7773015, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-8224909, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-942051, http://linkedlifedata.com/resource/pubmed/commentcorrection/10938354-9618461
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
123
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1363-74
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Lignification in transgenic poplars with extremely reduced caffeic acid O-methyltransferase activity.
pubmed:affiliation
Biologie Cellulaire, Institut National de la Recherche Agronomique, 78026 Versailles cedex, France. jouanin@versailles.inra.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't