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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5 Pt 1
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pubmed:dateCreated |
2000-9-7
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pubmed:abstractText |
Recombinant adenoviruses expressing human beta-glucuronidase (AxCAhGUS) and CTLA-4Ig (AxCACTLA-4Ig) were generated and therapeutic efficacy was investigated using a murine model of mucopolysaccharidosis type VII (MPSVII). Seven days after the intravenous administration of AxCAhGUS, high levels of beta-glucuronidase (GUSB) activity were observed in the liver, spleen, heart, lung, kidney, and serum, while viral DNA was predominantly detected in the liver. To investigate the contribution of in vivo cross-correction of GUSB between the liver and other organs, we injected the serum obtained from the transduced mice into untreated MPSVII mice. Similar distributions of GUSB activity were observed in the serum-injected mice, suggesting that GUSB activities detected in the extrahepatic organs were due to the cross-correction rather than the direct gene transduction. This result also suggested that maintaining high levels of GUSB in the systemic circulation was essential for the effective treatment of MPSVII. To achieve this, we injected AxCAhGUS and AxCACTLA-4Ig into MPSVII mice. Serum GUSB activity was sustained at high levels for more than 200 days and morphological normalization of the liver and spleen was observed for a year. This suggests that long-term therapeutic efficacy in visceral organs of MPSVII is achievable by coexpression of CTLA-4Ig through an in vivo cross-correction pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Ctla4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronidase,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoconjugates,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fc Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, IGF Type 2,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/abatacept,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1525-0016
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
406-13
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10933961-Adenoviridae,
pubmed-meshheading:10933961-Animals,
pubmed-meshheading:10933961-Antigens, CD,
pubmed-meshheading:10933961-Antigens, Differentiation,
pubmed-meshheading:10933961-CTLA-4 Antigen,
pubmed-meshheading:10933961-Cells, Cultured,
pubmed-meshheading:10933961-DNA, Viral,
pubmed-meshheading:10933961-DNA Primers,
pubmed-meshheading:10933961-Gene Expression,
pubmed-meshheading:10933961-Gene Therapy,
pubmed-meshheading:10933961-Genetic Vectors,
pubmed-meshheading:10933961-Genotype,
pubmed-meshheading:10933961-Glucuronidase,
pubmed-meshheading:10933961-Humans,
pubmed-meshheading:10933961-Immunoconjugates,
pubmed-meshheading:10933961-Immunoglobulin Fc Fragments,
pubmed-meshheading:10933961-Kidney,
pubmed-meshheading:10933961-Liver,
pubmed-meshheading:10933961-Lung,
pubmed-meshheading:10933961-Mice,
pubmed-meshheading:10933961-Mice, Inbred C57BL,
pubmed-meshheading:10933961-Mucopolysaccharidosis VII,
pubmed-meshheading:10933961-Receptor, IGF Type 2,
pubmed-meshheading:10933961-Recombinant Fusion Proteins,
pubmed-meshheading:10933961-Spleen,
pubmed-meshheading:10933961-beta-Galactosidase
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pubmed:year |
2000
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pubmed:articleTitle |
Adenovirus-mediated gene therapy for mucopolysaccharidosis VII: involvement of cross-correction in wide-spread distribution of the gene products and long-term effects of CTLA-4Ig coexpression.
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pubmed:affiliation |
Department of Genetics, National Children's Medical Research Center, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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