Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2001-1-9
pubmed:databankReference
pubmed:abstractText
We present a model for the skeletal muscle troponin-C (TnC)/troponin-I (TnI) interaction, a critical molecular switch that is responsible for calcium-dependent regulation of the contractile mechanism. Despite concerted efforts by multiple groups for more than a decade, attempts to crystallize troponin-C in complex with troponin-I, or in the ternary troponin-complex, have not yet delivered a high-resolution structure. Many groups have pursued different experimental strategies, such as X-ray crystallography, NMR, small-angle scattering, chemical cross-linking, and fluorescent resonance energy transfer (FRET) to gain insights into the nature of the TnC/TnI interaction. We have integrated the results of these experiments to develop a model of the TnC/TnI interaction, using an atomic model of TnC as a scaffold. The TnI sequence was fit to each of two alternate neutron scattering envelopes: one that winds about TnC in a left-handed sense (Model L), and another that winds about TnC in a right-handed sense (Model R). Information from crystallography and NMR experiments was used to define segments of the models. Tests show that both models are consistent with available cross-linking and FRET data. The inhibitory region TnI(95-114) is modeled as a flexible beta-hairpin, and in both models it is localized to the same region on the central helix of TnC. The sequence of the inhibitory region is similar to that of a beta-hairpin region of the actin-binding protein profilin. This similarity supports our model and suggests the possibility of using an available profilin/actin crystal structure to model the TnI/actin interaction. We propose that the beta-hairpin is an important structural motif that communicates the Ca2+-activated troponin regulatory signal to actin.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10082949, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10098965, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10220335, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10346913, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10350487, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10387074, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10506219, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10592235, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10600384, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10631988, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10651267, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-10677290, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-11004542, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-124731, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-134666, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-1445871, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-146828, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-179535, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-1856208, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-1960733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2026604, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2108719, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2138356, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2248948, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2402508, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2507540, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2542260, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2720128, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2775740, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-2971047, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3137220, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3196696, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3210231, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3338985, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3365370, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3427058, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3508279, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3743779, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3949740, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3974698, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-3986667, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-563468, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-7552750, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-7601340, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-7766602, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-7947685, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8031757, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8066087, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8089144, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8106506, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8119911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8130210, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8170919, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8180191, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8294472, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8413665, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8500614, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8527454, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8639710, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8780504, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8808585, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8815803, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8825738, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-8848832, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9017213, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9200712, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9283095, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9299323, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9353310, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9367759, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9438870, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9448267, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9497355, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9560191, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9635764, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9682135, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9710540, http://linkedlifedata.com/resource/pubmed/commentcorrection/10933496-9730814
pubmed:language
eng
More...