Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-22
pubmed:abstractText
All rearranging antigen receptor genes have one or two highly diverse complementarity determining regions (CDRs) among the six that typically form the ligand binding surface. We report here that, in the case of antibodies, diversity at one of these regions, CDR3 of the V(H) domain, is sufficient to permit otherwise identical IgM molecules to distinguish between a variety of hapten and protein antigens. Furthermore, we find that somatic mutation can allow such antibodies to achieve surprisingly high affinities. These results are consistent with a model in which the highly diverse CDR3 loops are the key determinant of specificity in antigen recognition in both T cell receptors (TCR) and antibodies, whereas the germline-encoded CDR1 and CDR2 sequences are much more cross-reactive.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/2,4-dinitrophenyl keyhole limpet..., http://linkedlifedata.com/resource/pubmed/chemical/Antigens, http://linkedlifedata.com/resource/pubmed/chemical/Complementarity Determining Regions, http://linkedlifedata.com/resource/pubmed/chemical/Dextrans, http://linkedlifedata.com/resource/pubmed/chemical/Haptens, http://linkedlifedata.com/resource/pubmed/chemical/Hemocyanin, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Heavy Chains, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin kappa-Chains, http://linkedlifedata.com/resource/pubmed/chemical/Ovalbumin, http://linkedlifedata.com/resource/pubmed/chemical/Polysaccharides, Bacterial, http://linkedlifedata.com/resource/pubmed/chemical/keyhole-limpet hemocyanin
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1074-7613
pubmed:author
pubmed:issnType
Print
pubmed:volume
13
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
37-45
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10933393-Amino Acid Sequence, pubmed-meshheading:10933393-Animals, pubmed-meshheading:10933393-Antibody Affinity, pubmed-meshheading:10933393-Antibody Diversity, pubmed-meshheading:10933393-Antibody Specificity, pubmed-meshheading:10933393-Antigens, pubmed-meshheading:10933393-Complementarity Determining Regions, pubmed-meshheading:10933393-Dextrans, pubmed-meshheading:10933393-Haptens, pubmed-meshheading:10933393-Hemocyanin, pubmed-meshheading:10933393-Humans, pubmed-meshheading:10933393-Immunoglobulin G, pubmed-meshheading:10933393-Immunoglobulin Heavy Chains, pubmed-meshheading:10933393-Immunoglobulin M, pubmed-meshheading:10933393-Immunoglobulin Variable Region, pubmed-meshheading:10933393-Immunoglobulin kappa-Chains, pubmed-meshheading:10933393-Mice, pubmed-meshheading:10933393-Mice, Inbred C57BL, pubmed-meshheading:10933393-Mice, Transgenic, pubmed-meshheading:10933393-Molecular Sequence Data, pubmed-meshheading:10933393-Ovalbumin, pubmed-meshheading:10933393-Polysaccharides, Bacterial, pubmed-meshheading:10933393-Sequence Analysis
pubmed:year
2000
pubmed:articleTitle
Diversity in the CDR3 region of V(H) is sufficient for most antibody specificities.
pubmed:affiliation
Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't