Linked Life Data

10932852

Source:http://linkedlifedata.com/resource/pubmed/id/10932852

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-8-30
pubmed:abstractText
The fluorogenic reagent 9-fluorenylmethyl chloroformate (FMOC) was evaluated for the derivatization of tertiary amphetamines prior to liquid chromatographic analysis. Conditions for the derivatization were investigated, including the reaction time, the derivatization reagent concentration and the pH, using N-methylephedrine as a model compound. On the basis of these studies, a method for the quantification of N-methylephedrine is presented. The method involves derivatization with FMOC at ambient temperature and separation of the derivatives formed on a LiChrospher C18, 5 microns, 125 x 4 mm id column using acetonitrile-water gradient elution. The proposed procedure shows good linearity, accuracy and reproducibility in the 1.0-25.0 micrograms mL-1 concentration range. The limit of detection was 0.1 microgram mL-1 and the limit of quantification was 0.5 microgram mL-1. The utility of the assay was demonstrated by determining N-methylephedrine in water and urine samples.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0003-2654
pubmed:author
pubmed:issnType
Print
pubmed:volume
125
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1071-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Derivatization of tertiary amphetamines with 9-fluorenylmethyl chloroformate for liquid chromatography: determination of N-methylephedrine.
pubmed:affiliation
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Spain.
pubmed:publicationType
Journal Article