Linked Life Data

10931942

Source:http://linkedlifedata.com/resource/pubmed/id/10931942

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17
pubmed:dateCreated
2000-9-19
pubmed:abstractText
The activity of glutamate transporters is essential for the temporal and spatial regulation of the neurotransmitter concentration in the synaptic cleft, and thus, is crucial for proper excitatory signaling. Initial steps in the process of glutamate transport take place within a time scale of microseconds to milliseconds. Here we compare the steady-state and pre-steady-state kinetics of the neuronal heterologously expressed glutamate transporter EAAC1, cloned from the mammalian retina. Rapid transporter dynamics, as measured by using whole-cell current recordings, were resolved by applying the laser-pulse photolysis technique of caged glutamate with a time resolution of 100 micros. EAAC1-mediated pre-steady-state currents are composed of two components: A transport current generated by substrate-coupled charge translocation across the membrane and an anion current that is not stoichiometrically coupled to glutamate transport. The two currents were temporally resolved and studied independently. Our results indicate a rapid glutamate-binding step occurring on a submillisecond time scale that precedes subsequent slower electrogenic glutamate translocation across the membrane within a few milliseconds. The voltage-dependent steady-state turnover time constant of the transporter is about 1/10 as fast, indicating that glutamate translocation is not rate limiting. A third process, the transition to an anion-conducting state, is delayed with respect to the onset of glutamate transport. These rapid transporter reaction steps are summarized in a sequential shuttle model that quantitatively accounts for the results obtained here and are discussed regarding their functional importance for glutamatergic neurotransmission in the central nervous system.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-10423421, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-10516269, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-10751425, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-1280334, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-1681832, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-2448470, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-2901670, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-6129891, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-6162960, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-708689, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7521911, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7546750, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7622462, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7748550, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7914198, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-7917301, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-8090718, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-8381026, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-8857541, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-8929335, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9011753, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9169528, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9394040, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9463476, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9736633, http://linkedlifedata.com/resource/pubmed/commentcorrection/10931942-9742136
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
97
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9706-11
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10931942-Amino Acid Transport System X-AG, pubmed-meshheading:10931942-Animals, pubmed-meshheading:10931942-Anions, pubmed-meshheading:10931942-Biological Transport, pubmed-meshheading:10931942-Carrier Proteins, pubmed-meshheading:10931942-Cell Line, pubmed-meshheading:10931942-Cell Membrane, pubmed-meshheading:10931942-Electric Conductivity, pubmed-meshheading:10931942-Excitatory Amino Acid Transporter 3, pubmed-meshheading:10931942-Glutamate Plasma Membrane Transport Proteins, pubmed-meshheading:10931942-Glutamic Acid, pubmed-meshheading:10931942-Humans, pubmed-meshheading:10931942-Kinetics, pubmed-meshheading:10931942-Lasers, pubmed-meshheading:10931942-Neurons, pubmed-meshheading:10931942-Photolysis, pubmed-meshheading:10931942-Potassium, pubmed-meshheading:10931942-Recombinant Proteins, pubmed-meshheading:10931942-Retina, pubmed-meshheading:10931942-Sodium, pubmed-meshheading:10931942-Symporters, pubmed-meshheading:10931942-Transfection
pubmed:year
2000
pubmed:articleTitle
Glutamate translocation of the neuronal glutamate transporter EAAC1 occurs within milliseconds.
pubmed:affiliation
Max-Planck-Institut für Biophysik, Frankfurt, Germany. grewer@mpibp-frankfurt.mpg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't