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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1975-7-14
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pubmed:abstractText |
A synthetic DNA fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase. The 3'-terminus of this elongated DNA strand was blocked with 2', 3'-dideoxyadenylate to prevent hydrolysis by the 3'-exonuclease function of E. coli DNA polymerase I. This elongated and 3'-blocked fragment was annealed to an oligomeric primer and used as a template for the synthesis of a complementary copy of the synthetic 19-mer. The product of such a repair synthesis was separated by gel filtration and analyzed by nearest neighbor techniques. All template strands were copied with complete repair in over 90% of the chains. Facile recovery of the elongated template by virtue of its size permitted repetition of the copy process, thus allowing accumulation of the desired strand.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-14114849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4312461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4336040,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4337512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4339879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4369229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4560009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4578443,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4734198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4762420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4890762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4927950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-4928649,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1093143-5543689
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
43-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1093143-Animals,
pubmed-meshheading:1093143-Cattle,
pubmed-meshheading:1093143-DNA,
pubmed-meshheading:1093143-DNA, Bacterial,
pubmed-meshheading:1093143-DNA Nucleotidyltransferases,
pubmed-meshheading:1093143-DNA Replication,
pubmed-meshheading:1093143-Escherichia coli,
pubmed-meshheading:1093143-Methods,
pubmed-meshheading:1093143-Nucleic Acid Hybridization,
pubmed-meshheading:1093143-Oligonucleotides,
pubmed-meshheading:1093143-Polynucleotide 5'-Hydroxyl-Kinase,
pubmed-meshheading:1093143-Templates, Genetic,
pubmed-meshheading:1093143-Thymus Gland
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pubmed:year |
1975
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pubmed:articleTitle |
Enzymatic multiplication of a chemically synthesized DNA fragment.
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pubmed:publicationType |
Journal Article
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