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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1975-7-28
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pubmed:abstractText |
The binding of lactose repressor to poly d(A-T) at low ionic strength has been investigated by heat denaturation. The poly d(A-T) melting is monitored by optical density and the protein melting by circular dichroism. From the modification of the poly d(A-T) melting curve we estimate a maximum binding ratio of about one tetrameric repressor to about 20 basic pairs. The repressor melting can be interpreted as a global shift from a to b structure of about 25 residues per subunit. The melting curves of poly d(A-T) and repressor have not a shape easy to interpret; nevertheless both show a cooperative transition in the same temperature range where we can evaluate that about 3.8 aminoacid residues shift from a to b structure when 1 bases pair melt.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
303-18
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1093136-Adenine Nucleotides,
pubmed-meshheading:1093136-Bacterial Proteins,
pubmed-meshheading:1093136-Binding Sites,
pubmed-meshheading:1093136-Circular Dichroism,
pubmed-meshheading:1093136-DNA, Bacterial,
pubmed-meshheading:1093136-Deoxyribonucleotides,
pubmed-meshheading:1093136-Escherichia coli,
pubmed-meshheading:1093136-Hot Temperature,
pubmed-meshheading:1093136-Kinetics,
pubmed-meshheading:1093136-Lactose,
pubmed-meshheading:1093136-Mutation,
pubmed-meshheading:1093136-Nucleic Acid Conformation,
pubmed-meshheading:1093136-Nucleic Acid Denaturation,
pubmed-meshheading:1093136-Polynucleotides,
pubmed-meshheading:1093136-Protein Binding,
pubmed-meshheading:1093136-Protein Conformation,
pubmed-meshheading:1093136-Spectrophotometry, Ultraviolet,
pubmed-meshheading:1093136-Thymine Nucleotides
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pubmed:year |
1975
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pubmed:articleTitle |
Binding of lactose repressor to poly d(A-T) : OD AND CD melting of the complex.
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pubmed:publicationType |
Journal Article
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