Source:http://linkedlifedata.com/resource/pubmed/id/10930736
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2000-9-12
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pubmed:abstractText |
An unusual genotype of Cryptosporidium was identified in the faeces of six human patients by PCR/RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene. Conventional microscopy showed oocysts indistinguishable in size from those of Cryptosporidium parvum, which reacted with two different commercially available anti-oocyst monoclonal antibodies. The isolates were further characterised by PCR/RFLP analysis of the thrombospondin-related adhesive protein of Cryptosporidium-1 (TRAP-C1) genes as well as by DNA sequencing of the COWP and the TRAP-C1 gene fragments and of two regions of the 18S rRNA gene. Sequence analysis of the COWP, TRAP-C1, and 18S rRNA gene fragments confirmed that this genotype is genetically distinct from C. parvum. 18S rRNA gene sequences were found to be identical to those published for Cryptosporidium meleagridis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
189
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-94
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10930736-Amino Acid Sequence,
pubmed-meshheading:10930736-Animals,
pubmed-meshheading:10930736-Cryptosporidium,
pubmed-meshheading:10930736-Feces,
pubmed-meshheading:10930736-Genes, Protozoan,
pubmed-meshheading:10930736-Genotype,
pubmed-meshheading:10930736-Humans,
pubmed-meshheading:10930736-Molecular Sequence Data
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pubmed:year |
2000
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pubmed:articleTitle |
The identification and characterisation of an unusual genotype of Cryptosporidium from human faeces as Cryptosporidium meleagridis.
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pubmed:affiliation |
Food Safety Microbiology Laboratory, Division of Gastrointestinal Infections, PHLS Central Public Health Laboratory, 61 Colindale Avenue, NW9 5HT, London, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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