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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-8-25
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pubmed:databankReference |
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pubmed:abstractText |
We describe the crystal structure of a complex between importin-beta residues 1-442 (Ib442) and five FxFG nucleoporin repeats from Nsp1p. Nucleoporin FxFG cores bind on the convex face of Ib442 to a primary site between the A helices of HEAT repeats 5 and 6, and to a secondary site between HEAT repeats 6 and 7. Mutations at importin-beta Ile178 in the primary FxFG binding site reduce both binding and nuclear protein import, providing direct evidence for the functional significance of the importin-beta-FxFG interaction. The FxFG binding sites on importin-beta do not overlap with the RanGTP binding site. Instead, RanGTP may release importin-beta from FxFG nucleoporins by generating a conformational change that alters the structure of the FxFG binding site.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
102
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
99-108
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10929717-Amino Acid Sequence,
pubmed-meshheading:10929717-Animals,
pubmed-meshheading:10929717-Binding Sites,
pubmed-meshheading:10929717-Biological Transport,
pubmed-meshheading:10929717-Calcium-Binding Proteins,
pubmed-meshheading:10929717-Cell Nucleus,
pubmed-meshheading:10929717-Crystallography, X-Ray,
pubmed-meshheading:10929717-Fungal Proteins,
pubmed-meshheading:10929717-HeLa Cells,
pubmed-meshheading:10929717-Humans,
pubmed-meshheading:10929717-Karyopherins,
pubmed-meshheading:10929717-Models, Molecular,
pubmed-meshheading:10929717-Molecular Sequence Data,
pubmed-meshheading:10929717-Mutagenesis,
pubmed-meshheading:10929717-Nuclear Pore Complex Proteins,
pubmed-meshheading:10929717-Nuclear Proteins,
pubmed-meshheading:10929717-Protein Structure, Secondary,
pubmed-meshheading:10929717-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:10929717-Sequence Homology, Amino Acid,
pubmed-meshheading:10929717-Structure-Activity Relationship,
pubmed-meshheading:10929717-Tandem Repeat Sequences,
pubmed-meshheading:10929717-ran GTP-Binding Protein
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pubmed:year |
2000
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pubmed:articleTitle |
Structural basis for the interaction between FxFG nucleoporin repeats and importin-beta in nuclear trafficking.
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pubmed:affiliation |
MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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