|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
2000-8-22
|
|
pubmed:abstractText |
Faeces of immunocompromised patients are often contaminated with the chitin-containing spores of microsporidia and Candida, which exclude the use of the chitin-specific fluorescent brightener Calcofluor white M2R for the identification of microsporidian spores. We developed a combination staining of Calcofluor white M2R with modified trichrome-blue staining and subsequent methylene-blue incubation which permits discrimination between these two types of spores. As a basis for diagnosis, a difference in the fluorescence pattern (365-440 nm) is combined with a difference in the light microscopic staining pattern. Under fluorescence conditions microsporidia spores have a spotted, brilliant white Calcofluor fluorescence and can easily be identified, while Candida spores show a reddish purple colour. Under the light microscope microsporidian spores show a light red colour with nonstained vacuole spots or strips in contrast to the yeast spores with their red-brown colour. This combination technique offers a highly specific means for the diagnosis of microsporidia spores in faeces.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jun
|
|
pubmed:issn |
1360-2276
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
453-8
|
|
pubmed:dateRevised |
2006-11-7
|
|
pubmed:meshHeading |
pubmed-meshheading:10929147-Animals,
pubmed-meshheading:10929147-Azo Compounds,
pubmed-meshheading:10929147-Benzenesulfonates,
pubmed-meshheading:10929147-Candida albicans,
pubmed-meshheading:10929147-Candidiasis,
pubmed-meshheading:10929147-Cercopithecus aethiops,
pubmed-meshheading:10929147-Eosine Yellowish-(YS),
pubmed-meshheading:10929147-Feces,
pubmed-meshheading:10929147-Fluorescent Dyes,
pubmed-meshheading:10929147-Humans,
pubmed-meshheading:10929147-Immunocompromised Host,
pubmed-meshheading:10929147-Macaca mulatta,
pubmed-meshheading:10929147-Methyl Green,
pubmed-meshheading:10929147-Methylene Blue,
pubmed-meshheading:10929147-Microsporida,
pubmed-meshheading:10929147-Microsporidiosis,
pubmed-meshheading:10929147-Sensitivity and Specificity,
pubmed-meshheading:10929147-Spores,
pubmed-meshheading:10929147-Staining and Labeling,
pubmed-meshheading:10929147-Vero Cells
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Microsporidia and Candida spores: their discrimination by Calcofluor, trichrome-blue and methylene-blue combination staining.
|
|
pubmed:affiliation |
Department of Parasitology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
|
|
pubmed:publicationType |
Journal Article
|
