Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1975-7-23
pubmed:abstractText
Two of seven sucrose-fermenting Salmonella strains obtained from clinical sources were found capable of conjugal transfer of the sucrose fermentation (Scr+) property to the Escherichia coli K-12 strain WR3026. The genetic elements conferring this Scr+ property, designated scr-53 and scr-94, were then conjugally transmissible from Escherichia coli WR3026 Scr+ exconjugants to other strains of Escherichia coli at frequences of 5 times 10- minus 6 to 5 times 10- minus 3 for the scr-53 element and 10- minus 6 to 10- minus 5 for the scr-94 element. In Escherichia coli hosts, both of these elements were compatible with F-lac and with each of six previously characterized transmissible lac elements. No antibiotic resistance characteristics or colicin production were discovered to be associated with either scr-53 or scr-94. Neither scr element generated a male host response to the female-specific phage phiII, but the scr-53 element rendered its Escherichia coli host sensitive to the male-specific phage R-17. Escherichia coli hosts containing scr-53 were susceptible to lysis by P1vir, and transduction of the scr-53 element was accomplished with this phage. The scr-53 element was isolated from Escherichia coli WR3026, Scr+ transductants, and Escherichia coli WR2036 Scr+ exconjugants as a covalently closed circular deoxyribonucleic acid molecule with a molecular weight (determined by electron microscopy) of approximately 52 times 10-6. Receipt of the scr-94 element rendered Escherichia coli hosts of this element unsusceptible to lysis by P1vir, although adsorption of the phage by an Escherichia coli WR3026 exconjugant containing scr-94 occurred as efficiently as it did on WR3026 itself. Repeated examination of Escherichia coli strains harboring scr-94, as well as of the Salmonella strain which initially contained it, did not reveal the presence of circular deoxyribonucleic acid. The synthesis of the sucrose cleaving enzyme was inducible in Escherichia coli exconjugants containing either scr-53 or scr-94.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-14202287, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-14240944, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-14267285, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-16561959, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4564488, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4579875, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4584803, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4600107, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4818194, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4898999, http://linkedlifedata.com/resource/pubmed/commentcorrection/1092649-4945199
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
401-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains.
pubmed:publicationType
Journal Article