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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-9-14
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pubmed:databankReference |
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pubmed:abstractText |
Little is known regarding the ligand specificity of Ly-49 activating receptor subfamily members expressed by NK cells. A new Ly-49 activating receptor related to Ly-49A in its extracellular domain, designated Ly-49P, was recently cloned from 129 strain mice. We independently cloned an apparent allele of Ly-49P expressed by nonobese diabetic and nonobese diabetes-resistant mouse strain NK cells. We found it to be reactive with the A1 Ab thought to recognize a polymorphic epitope expressed only by the Ly-49A inhibitory receptor of the C57BL/6 strain. Rat RNK-16 cells transfected with Ly-49P mediated reverse Ab-dependent cellular cytotoxicity of FcR-positive target cells, indicating that Ly-49P can activate NK-mediated lysis. We determined that RNK-16 lysis of Con A blasts induced by Ly-49P was MHC dependent, resulting in efficient lysis of H-2Dd-bearing targets. We found that the Dd alpha1/alpha2 domain is required for Ly-49P-mediated RNK-16 activation, as determined by exon shuffling and transfection. Thus, Ly-49P is the second activating Ly-49 receptor demonstrated to induce NK cytotoxicity by recognizing a class I MHC molecule.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/H-2 Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Immune Sera,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Ly49P receptor,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, NK Cell Lectin-Like,
http://linkedlifedata.com/resource/pubmed/chemical/histocompatibility antigen H-2D(b)
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1771-81
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10925254-Amino Acid Sequence,
pubmed-meshheading:10925254-Animals,
pubmed-meshheading:10925254-Antigens, Ly,
pubmed-meshheading:10925254-Carrier Proteins,
pubmed-meshheading:10925254-Concanavalin A,
pubmed-meshheading:10925254-Cytotoxicity, Immunologic,
pubmed-meshheading:10925254-Epitopes,
pubmed-meshheading:10925254-Female,
pubmed-meshheading:10925254-H-2 Antigens,
pubmed-meshheading:10925254-Immune Sera,
pubmed-meshheading:10925254-Killer Cells, Natural,
pubmed-meshheading:10925254-Lectins, C-Type,
pubmed-meshheading:10925254-Lymphocyte Activation,
pubmed-meshheading:10925254-Major Histocompatibility Complex,
pubmed-meshheading:10925254-Membrane Proteins,
pubmed-meshheading:10925254-Mice,
pubmed-meshheading:10925254-Mice, Inbred BALB C,
pubmed-meshheading:10925254-Mice, Inbred C57BL,
pubmed-meshheading:10925254-Mice, Inbred NOD,
pubmed-meshheading:10925254-Molecular Sequence Data,
pubmed-meshheading:10925254-Protein Structure, Tertiary,
pubmed-meshheading:10925254-Rats,
pubmed-meshheading:10925254-Rats, Inbred F344,
pubmed-meshheading:10925254-Receptors, Immunologic,
pubmed-meshheading:10925254-Receptors, NK Cell Lectin-Like,
pubmed-meshheading:10925254-Sequence Homology, Amino Acid,
pubmed-meshheading:10925254-Species Specificity,
pubmed-meshheading:10925254-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Ly-49P activates NK-mediated lysis by recognizing H-2Dd.
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pubmed:affiliation |
Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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