Source:http://linkedlifedata.com/resource/pubmed/id/10924092
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2000-9-27
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| pubmed:abstractText |
The Bcl-2 family of proteins has been shown to play a central role in the regulation of apoptosis. We have examined the expression of several Bcl-2 homologs upon stimulation of CD34(+) human hematopoietic progenitor cells. CD34(+) cells were induced to differentiate into predominantly erythroid cells in the presence of erythropoietin (Epo) and stem cell factor (SCF), while the addition of G-CSF and SCF led to differentiation predominantly into granulocytic cells, as demonstrated by immunophenotyping and morphological examination of cultured cells. In Epo- and SCF-stimulated cells, we found a marked increase in the level of Bcl-x(L) protein expression and downregulation of Bax expression, apparent from day 4 and more pronounced on days 8 and 21. In contrast, Bcl-x(L) protein expression was downregulated in G-CSF- and SCF-stimulated cells compared with cells cultured in medium alone, whereas there was no sign of change in the level of Bax. Mcl-1 expression showed a biphasic expression pattern in both early erythropoiesis and early granulopoiesis, but with an inverse regulation. Thus, Mcl-1 levels initially decreased in granulocytic progenitor cells and increased in erythroid progenitor cells. Finally, Bcl-2 expression was significantly downregulated in both Epo and SCF and G-CSF- and SCF-stimulated cells. The role of the distinct upregulation of Bcl-x(L) in early erythroid differentiation was further examined by use of specific ribozymes against Bcl-x(L). Addition of Bcl-x(L) ribozymes promoted a clear increase in cell death of Epo- and SCF-stimulated cells, while erythroid differentiation was not affected. In conclusion, we found a distinct regulation of several Bcl-2 family members in CD34(+) cells dependent on the cytokine stimulation given. The use of Bcl-x(L)-specific ribozymes suggested that Bcl-x(L) is important for survival but not for differentiation of erythroid progenitor cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/BAX protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/BCL2L1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Erythropoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte Colony-Stimulating...,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-bcl-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Catalytic,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-2-Associated X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/bcl-X Protein,
http://linkedlifedata.com/resource/pubmed/chemical/myeloid cell leukemia sequence 1...
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| pubmed:status |
MEDLINE
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| pubmed:issn |
1066-5099
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
18
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
261-72
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10924092-Adult,
pubmed-meshheading:10924092-Antigens, CD34,
pubmed-meshheading:10924092-Biological Markers,
pubmed-meshheading:10924092-Cell Differentiation,
pubmed-meshheading:10924092-Cell Lineage,
pubmed-meshheading:10924092-Cell Survival,
pubmed-meshheading:10924092-Cells, Cultured,
pubmed-meshheading:10924092-Erythroid Precursor Cells,
pubmed-meshheading:10924092-Erythropoietin,
pubmed-meshheading:10924092-Granulocyte Colony-Stimulating Factor,
pubmed-meshheading:10924092-Granulocytes,
pubmed-meshheading:10924092-Hematopoietic Stem Cells,
pubmed-meshheading:10924092-Humans,
pubmed-meshheading:10924092-Neoplasm Proteins,
pubmed-meshheading:10924092-Proto-Oncogene Proteins,
pubmed-meshheading:10924092-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:10924092-RNA, Catalytic,
pubmed-meshheading:10924092-Stem Cell Factor,
pubmed-meshheading:10924092-bcl-2-Associated X Protein,
pubmed-meshheading:10924092-bcl-X Protein
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| pubmed:year |
2000
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| pubmed:articleTitle |
Differential expression of bcl-2 homologs in human CD34(+) hematopoietic progenitor cells induced to differentiate into erythroid or granulocytic cells.
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| pubmed:affiliation |
Department of Immunology, Institute for Cancer Research, The Norwegian Radium Hospital, Montebello, Oslo, Norway. dag.josefsen@labmed.uio.no
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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