|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
15
|
|
pubmed:dateCreated |
2000-9-28
|
|
pubmed:abstractText |
Rice expressing the Pi-ta gene is resistant to strains of the rice blast fungus, Magnaporthe grisea, expressing AVR-Pita in a gene-for-gene relationship. Pi-ta encodes a putative cytoplasmic receptor with a centrally localized nucleotide-binding site and leucine-rich domain (LRD) at the C-terminus. AVR-Pita is predicted to encode a metalloprotease with an N-terminal secretory signal and pro-protein sequences. AVR-Pita(176) lacks the secretory and pro-protein sequences. We report here that transient expression of AVR-Pita(176) inside plant cells results in a Pi-ta-dependent resistance response. AVR-Pita(176) protein is shown to bind specifically to the LRD of the Pi-ta protein, both in the yeast two-hybrid system and in an in vitro binding assay. Single amino acid substitutions in the Pi-ta LRD or in the AVR-Pita(176) protease motif that result in loss of resistance in the plant also disrupt the physical interaction, both in yeast and in vitro. These data suggest that the AVR-Pita(176) protein binds directly to the Pi-ta LRD region inside the plant cell to initiate a Pi-ta-mediated defense response.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-10334981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-10369664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-10437829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-1837147,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-1863778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-2016048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-2050124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-2547163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-3002240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7549480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7556057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7566127,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7638602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7859515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7902614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7923358,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-7954825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8086433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8114941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8264799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8608599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8689679,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8768370,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8980236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-8986840,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9014365,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9144966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9263447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9596635,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9734361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9853621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10921881-9861059
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0261-4189
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
19
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
4004-14
|
|
pubmed:dateRevised |
2010-9-14
|
|
pubmed:meshHeading |
pubmed-meshheading:10921881-Binding Sites,
pubmed-meshheading:10921881-Biolistics,
pubmed-meshheading:10921881-Gene Transfer Techniques,
pubmed-meshheading:10921881-Genes, Fungal,
pubmed-meshheading:10921881-Genes, Plant,
pubmed-meshheading:10921881-Magnaporthe,
pubmed-meshheading:10921881-Metalloendopeptidases,
pubmed-meshheading:10921881-Oryza sativa,
pubmed-meshheading:10921881-Plant Diseases,
pubmed-meshheading:10921881-Plant Leaves,
pubmed-meshheading:10921881-Plant Proteins,
pubmed-meshheading:10921881-Protein Binding,
pubmed-meshheading:10921881-Receptors, Cytoplasmic and Nuclear,
pubmed-meshheading:10921881-Two-Hybrid System Techniques
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Direct interaction of resistance gene and avirulence gene products confers rice blast resistance.
|
|
pubmed:affiliation |
DuPont Agricultural Products, PO Box 80402, Wilmington, DE 19880-0402, USA.
|
|
pubmed:publicationType |
Journal Article
|
