Source:http://linkedlifedata.com/resource/pubmed/id/10920222
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-9-25
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| pubmed:abstractText |
The effects of mechanical loading on the osteoblast phenotype remain unclear because of many variables inherent to the current experimental models. This study reports on utilization of a mouse tooth movement model and a semiquantitative video image analysis of in situ hybridization to determine the effect of mechanical loading on cell-specific expression of type I collagen (collagen I) and alkaline phosphatase (ALP) genes in periodontal osteoblasts, using nonosseous cells as an internal standard. The histomorphometric analysis showed intense osteoid deposition after 3 days of treatment, confirming the osteoinductive nature of the mechanical signal. The results of in situ hybridization showed that in control periodontal sites both collagen I and ALP mRNAs were expressed uniformly across the periodontium. Treatment for 24 hours enhanced the ALP mRNA level about twofold over controls and maintained that level of stimulation after 6 days. In contrast, collagen I mRNA level was not affected after 24 hours of treatment, but it was stimulated 2.8-fold at day 6. This increase reflected enhanced gene expression in individual osteoblasts, since the increase in osteoblast number was small. These results indicate that (1) the mouse model and a semiquantitative video image analysis are suitable for detecting osteoblast-specific gene regulation by mechanical loading; (2) osteogenic mechanical stress induces deposition of bone matrix primarily by stimulating differentiation of osteoblasts, and, to a lesser extent, by an increase in number of these cells; (3) ALP is an early marker of mechanically-induced differentiation of osteoblasts. (4) osteogenic mechanical stimulation in vivo produces a cell-specific 2.8-fold increase in collagen gene expression in mature, matrix-depositing osteoblasts located on the bone surface and within the osteoid layer.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
0171-967X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
67
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
163-72
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:10920222-Alkaline Phosphatase,
pubmed-meshheading:10920222-Animals,
pubmed-meshheading:10920222-Cell Count,
pubmed-meshheading:10920222-Cell Differentiation,
pubmed-meshheading:10920222-Collagen,
pubmed-meshheading:10920222-Dental Stress Analysis,
pubmed-meshheading:10920222-In Situ Hybridization,
pubmed-meshheading:10920222-Mice,
pubmed-meshheading:10920222-Osteoblasts,
pubmed-meshheading:10920222-Periodontium,
pubmed-meshheading:10920222-RNA, Messenger,
pubmed-meshheading:10920222-Stress, Mechanical,
pubmed-meshheading:10920222-Time Factors
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| pubmed:year |
2000
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| pubmed:articleTitle |
Mechanical loading stimulates differentiation of periodontal osteoblasts in a mouse osteoinduction model: effect on type I collagen and alkaline phosphatase genes.
|
| pubmed:affiliation |
Departments of Orthodontics and Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 78284-7910, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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