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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
29
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pubmed:dateCreated |
2000-8-16
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pubmed:abstractText |
The c-myc gene is frequently over-expressed in human cancers and is involved in regulation of proliferation, differentiation and apoptosis. c-Myc is a transcription factor that acts primarily by regulating the expression of other genes. However, it has been very difficult to identify bona fide c-Myc target genes that explain its diverse biological activities. The recent generation of c-myc deficient Rat1A fibroblasts with a profound and stable growth defect provides a new system to search for genes that can substitute for c-myc in proliferation. In this study, we have attempted to identify genes that rescue the slow growth phenotype of c-myc null cells through introduction of a series of potent cell cycle regulatory genes and several retroviral cDNA expression libraries. None of the candidate genes tested, including SV40 T-antigen and adenovirus E1A, caused reversal of the c-myc null growth defect. Furthermore, extensive screens with high-complexity retroviral cDNA libraries from three different tissue sources revealed that only c-myc and N-myc rescued the c-myc null slow-growth phenotype. Our data support the notion that there are no functional equivalents of the myc family of proto-oncogenes and also suggest that there are no c-Myc-activated genes that alone can substitute for c-Myc in control of cell proliferation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenovirus E1A Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Arid4a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin D1,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin E,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E2F Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/E2F2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/E2F3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/E2F3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/E2f3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/Retinoblastoma-Binding Protein 1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor DP1,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
19
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3330-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10918589-Adenovirus E1A Proteins,
pubmed-meshheading:10918589-Animals,
pubmed-meshheading:10918589-Antigens, Polyomavirus Transforming,
pubmed-meshheading:10918589-Carrier Proteins,
pubmed-meshheading:10918589-Cell Cycle Proteins,
pubmed-meshheading:10918589-Cell Division,
pubmed-meshheading:10918589-Cell Line,
pubmed-meshheading:10918589-Cyclin D1,
pubmed-meshheading:10918589-Cyclin E,
pubmed-meshheading:10918589-DNA-Binding Proteins,
pubmed-meshheading:10918589-E2F Transcription Factors,
pubmed-meshheading:10918589-E2F2 Transcription Factor,
pubmed-meshheading:10918589-E2F3 Transcription Factor,
pubmed-meshheading:10918589-Fibroblasts,
pubmed-meshheading:10918589-Helix-Loop-Helix Motifs,
pubmed-meshheading:10918589-Humans,
pubmed-meshheading:10918589-K562 Cells,
pubmed-meshheading:10918589-Leucine Zippers,
pubmed-meshheading:10918589-Mice,
pubmed-meshheading:10918589-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:10918589-Rats,
pubmed-meshheading:10918589-Retinoblastoma-Binding Protein 1,
pubmed-meshheading:10918589-Transcription Factor DP1,
pubmed-meshheading:10918589-Transcription Factors
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pubmed:year |
2000
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pubmed:articleTitle |
A genetic screen to identify genes that rescue the slow growth phenotype of c-myc null fibroblasts.
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pubmed:affiliation |
Division of Molecular Carcinogenesis, and Center for Biomedical Genetics, The Netherlands Cancer Institute, Amsterdam.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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