| pubmed-article:1091543 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0030705 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0237401 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0237868 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0023434 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0205307 | lld:lifeskim |
| pubmed-article:1091543 | lifeskim:mentions | umls-concept:C0036679 | lld:lifeskim |
| pubmed-article:1091543 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:1091543 | pubmed:dateCreated | 1975-6-29 | lld:pubmed |
| pubmed-article:1091543 | pubmed:abstractText | Previous studies have shown that lymphocytes from patients with chronic lymphocytic leukaemia have a diminished response to mitogens which stimulate T cells. Chronic lymphocytic leukaemia is most often a disease of accumulating B cells so that T lymphocytes are diluted by large numbers of leukaemic cells. Direct comparison with the responses of normal lymphocytes to mitogenic stimulation is therefore suspect. To circumvent this difficulty, a method of isolating T cells from normal individuals and patients with chronic lymphocytic leukaemia was developed. Lymphocytes containing an average of 16.1 per cent B cells from normal individuals were applied to IgG-anti-IgG-coated Degalan bead columns and held at 4 degrees for 2 hours. The eluted cells contained less than 2 per cent B cells. When chronic lymphocytic leukaemic lymphocytes, containing an average of 68.6 per cent B cells, were applied to IgG-anti-IgG columns, the eluted cells contained 36.4 per cent B cells. To improve the purification of T lymphocytes, columns of uncoated Degalan beads were used to remove non-specifically adherent cells. Eluted lymphocytes were then applied to IgG-anti-IgG columns. This resulted in the recovery of purified populations of T cells with less than 2 per cent contamination with B cells. Patients with chronic lymphocytic leukaemia were found to have lymphocytes with either a normal density or a low density of surface immunoglobulins. B cells were successfully removed from lymphocyte suspensions in all cases of chronic lymphocytic leukaemia with a normal density of lymphocyte surface immunoglobulins. In the three cases of chronic lymphocytic leukaemia with low density surface immunoglobulins, separation by this method was unsuccessful. However, an enriched T-cell population was obtained when leukaemic lymphocytes which had lost all detectable surface immunoglobulins were passed through a column coated with heat-aggregated IgG. | lld:pubmed |
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| pubmed-article:1091543 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1091543 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1091543 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:1091543 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1091543 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:1091543 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:1091543 | pubmed:author | pubmed-author:FernandezL... | lld:pubmed |
| pubmed-article:1091543 | pubmed:author | pubmed-author:LangleyG RGR | lld:pubmed |
| pubmed-article:1091543 | pubmed:author | pubmed-author:MacSweenJ MJM | lld:pubmed |
| pubmed-article:1091543 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1091543 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:1091543 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1091543 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1091543 | pubmed:pagination | 231-41 | lld:pubmed |
| pubmed-article:1091543 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:1091543 | pubmed:year | 1975 | lld:pubmed |
| pubmed-article:1091543 | pubmed:articleTitle | Separation of T lymphocytes from normal individuals and patients with B lymphocyte chronic lymphocytic leukaemia. | lld:pubmed |
| pubmed-article:1091543 | pubmed:publicationType | Journal Article | lld:pubmed |
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