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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1975-6-29
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pubmed:abstractText |
Previous studies have shown that lymphocytes from patients with chronic lymphocytic leukaemia have a diminished response to mitogens which stimulate T cells. Chronic lymphocytic leukaemia is most often a disease of accumulating B cells so that T lymphocytes are diluted by large numbers of leukaemic cells. Direct comparison with the responses of normal lymphocytes to mitogenic stimulation is therefore suspect. To circumvent this difficulty, a method of isolating T cells from normal individuals and patients with chronic lymphocytic leukaemia was developed. Lymphocytes containing an average of 16.1 per cent B cells from normal individuals were applied to IgG-anti-IgG-coated Degalan bead columns and held at 4 degrees for 2 hours. The eluted cells contained less than 2 per cent B cells. When chronic lymphocytic leukaemic lymphocytes, containing an average of 68.6 per cent B cells, were applied to IgG-anti-IgG columns, the eluted cells contained 36.4 per cent B cells. To improve the purification of T lymphocytes, columns of uncoated Degalan beads were used to remove non-specifically adherent cells. Eluted lymphocytes were then applied to IgG-anti-IgG columns. This resulted in the recovery of purified populations of T cells with less than 2 per cent contamination with B cells. Patients with chronic lymphocytic leukaemia were found to have lymphocytes with either a normal density or a low density of surface immunoglobulins. B cells were successfully removed from lymphocyte suspensions in all cases of chronic lymphocytic leukaemia with a normal density of lymphocyte surface immunoglobulins. In the three cases of chronic lymphocytic leukaemia with low density surface immunoglobulins, separation by this method was unsuccessful. However, an enriched T-cell population was obtained when leukaemic lymphocytes which had lost all detectable surface immunoglobulins were passed through a column coated with heat-aggregated IgG.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-13770744,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4104288,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4106607,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4109700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4114496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4118467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4302696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4325203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4501203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4504846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4506240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4540126,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4540711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4541301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4542158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4556902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4567307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4577289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4600199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4627539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4628444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4705614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-4999540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-5063974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-5259376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-5276287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-5477925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1091543-5794114
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
231-41
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1091543-Animals,
pubmed-meshheading:1091543-Antibodies, Anti-Idiotypic,
pubmed-meshheading:1091543-B-Lymphocytes,
pubmed-meshheading:1091543-Cell Membrane,
pubmed-meshheading:1091543-Cell Separation,
pubmed-meshheading:1091543-Fluorescence,
pubmed-meshheading:1091543-Hot Temperature,
pubmed-meshheading:1091543-Humans,
pubmed-meshheading:1091543-Immune Adherence Reaction,
pubmed-meshheading:1091543-Immunoglobulin G,
pubmed-meshheading:1091543-Immunoglobulin M,
pubmed-meshheading:1091543-Immunologic Techniques,
pubmed-meshheading:1091543-Leukemia, Lymphoid,
pubmed-meshheading:1091543-Rabbits,
pubmed-meshheading:1091543-Receptors, Antigen, B-Cell,
pubmed-meshheading:1091543-Sheep,
pubmed-meshheading:1091543-T-Lymphocytes
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pubmed:year |
1975
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pubmed:articleTitle |
Separation of T lymphocytes from normal individuals and patients with B lymphocyte chronic lymphocytic leukaemia.
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pubmed:publicationType |
Journal Article
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