Linked Life Data

10913365

Source:http://linkedlifedata.com/resource/pubmed/id/10913365

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-8-24
pubmed:abstractText
In this paper we report the cloning and sequencing of two small plasmids, pTAUp and pTADw, from the Antarctic Gram-negative Psychrobacter sp strain TA144. The observation that pTAUp contains a putative Rep-coding gene (Psyrep) suggested that its duplication occurs via a rolling-circle replication mechanism. This hypothesis was confirmed by the identification of the pTAUp single-stranded DNA form. The putative pTAUp plus origin of replication was found at the 3' end of the Psyrep by using an in vivo complementation assay. Structural similarities at the level of (i) gene organization, (ii) protein sequence, and (iii) nick site sequences strongly suggest that the psychrophilic enzyme belongs to a new subfamily of replication enzymes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
488-95
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10913365-Amino Acid Sequence, pubmed-meshheading:10913365-Antarctic Regions, pubmed-meshheading:10913365-Bacterial Proteins, pubmed-meshheading:10913365-Base Sequence, pubmed-meshheading:10913365-Cloning, Molecular, pubmed-meshheading:10913365-Codon, Initiator, pubmed-meshheading:10913365-DNA, Bacterial, pubmed-meshheading:10913365-DNA, Circular, pubmed-meshheading:10913365-DNA, Single-Stranded, pubmed-meshheading:10913365-DNA Helicases, pubmed-meshheading:10913365-DNA-Binding Proteins, pubmed-meshheading:10913365-Gammaproteobacteria, pubmed-meshheading:10913365-Genetic Complementation Test, pubmed-meshheading:10913365-Plasmids, pubmed-meshheading:10913365-Sequence Analysis, DNA, pubmed-meshheading:10913365-Sequence Homology, Amino Acid, pubmed-meshheading:10913365-Trans-Activators
pubmed:year
2000
pubmed:articleTitle
A rolling-circle plasmid from Psychrobacter sp. TA144: evidence for a novel rep subfamily.
pubmed:affiliation
Dipartimento di Chimica Organica e Biologica, University of Naples Federico II, via Mezzocannone, Naples, 16 80134, Italy. tutino@unina.it
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't