Linked Life Data

1091286

Source:http://linkedlifedata.com/resource/pubmed/id/1091286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1975-6-25
pubmed:abstractText
The isolation of six menaquinone mutants of Escherichia coli is described. It was shown that the mutants fall into two genetic classes. The first class carries mutations in a gene designated menA, which was located at minute 78 on the E. coli chromosome by cotransduction with the glpK and metB genes. The second class carries mutations in a gene designated menB. It was shown that this gene was not cotransducible with the menA gene. The biosynthesis of menaquinone in E. coli was studied using a variety of mutants blocked in aromatic biosynthesis together with the two classes of menaquinone mutants. It was demonstrated that chorismate is the branch point compound leading to menaquinone, and that 2-succinylbenzoic acid and 1,4-dihydroxy-2-naphthoic acid can serve as menaquinoone precursors in E. coli. It was also shown that menA- and menB- strains accumulate 1,4-dihydroxy-2-naphthoic acid and 2-succinylbenzoic acid, respectively, in their culture supernatants. The accumulation of the two compounds by the mutants together with their activity as menaquinone precursors provide strong evidence that they ar true intermediates in menaquinone biosynthesis. A pathway is proposed for the biosynthesis of bacterial menaquinones in which each intermediate has been adequately characterized.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
399-406
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Biosynthesis of bacterial menaquinones. Menaquinone mutants of Escherichia coli.
pubmed:publicationType
Journal Article