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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-10-5
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pubmed:abstractText |
Frizzled proteins act as putative Wnt receptors and depending on Wnt/Frizzled interactions distinct intracellular pathways can be activated. The canonical Wnt pathway, triggered by Wnt-1-type ligands, is the best characterized and involves the activation of the cytoplasmic protein Dishevelled (Dsh). The Xenopus frizzled 7 receptor (Xfz7) can act in the canonical (Wnt-1-type) as well as in a non-canonical Wnt pathway which involves the activation of protein kinase C (PKC). In order to analyze the interaction between Xfz7 and Xdsh protein, we tested the effect of overexpression of Xfz7 on the subcellular distribution of Xdsh-myc protein. We demonstrate that Xfz7 can recruit Xdsh-myc to the plasma membrane and target genes of the Wnt-1-type pathway such as siamois and nodal related 3 (Xnr-3) are only activated in the presence of exogenous Xdsh-myc. Xfz7 in combination with Xwnt-8b, however, is able to induce siamois even in the absence of Xdsh-myc. Similar results were obtained after expression of Human frizzled 5 (Hfz5) together with Xwnt-5a but this receptor-ligand combination recruits Xdsh-myc only very poorly to the membrane. These results suggests that the endogenous Xdsh pool and exogenous Xdsh-myc differ in their ability to be recruited by Frizzled receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/FZD5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/FZD7 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Frizzled Receptors,
http://linkedlifedata.com/resource/pubmed/chemical/Fzd7 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurotransmitter,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/WNT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt-1 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Wnt1 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Xnr3 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Zebrafish Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/dishevelled proteins,
http://linkedlifedata.com/resource/pubmed/chemical/siamois protein, Xenopus
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1058-8388
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
218
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
671-80
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10906785-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10906785-Animals,
pubmed-meshheading:10906785-Cell Membrane,
pubmed-meshheading:10906785-Fluorescent Antibody Technique,
pubmed-meshheading:10906785-Frizzled Receptors,
pubmed-meshheading:10906785-Homeodomain Proteins,
pubmed-meshheading:10906785-Humans,
pubmed-meshheading:10906785-Ligands,
pubmed-meshheading:10906785-Models, Biological,
pubmed-meshheading:10906785-Mutation,
pubmed-meshheading:10906785-Phosphoproteins,
pubmed-meshheading:10906785-Protein Binding,
pubmed-meshheading:10906785-Protein Kinase C,
pubmed-meshheading:10906785-Proto-Oncogene Proteins,
pubmed-meshheading:10906785-RNA,
pubmed-meshheading:10906785-Receptors, Cell Surface,
pubmed-meshheading:10906785-Receptors, G-Protein-Coupled,
pubmed-meshheading:10906785-Receptors, Neurotransmitter,
pubmed-meshheading:10906785-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10906785-Signal Transduction,
pubmed-meshheading:10906785-Transforming Growth Factor beta,
pubmed-meshheading:10906785-Wnt Proteins,
pubmed-meshheading:10906785-Wnt1 Protein,
pubmed-meshheading:10906785-Xenopus,
pubmed-meshheading:10906785-Xenopus Proteins,
pubmed-meshheading:10906785-Zebrafish Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Interaction of Frizzled 7 and Dishevelled in Xenopus.
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pubmed:affiliation |
Department of Cell Biology, Max-Planck Institute for Developmental Biology, Tübingen, Germany.
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pubmed:publicationType |
Journal Article
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