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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
40
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pubmed:dateCreated |
2000-10-23
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pubmed:databankReference |
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pubmed:abstractText |
A 130-kDa glycoprotein (p130) has been found to be associated with surrogate light chain on pro- and pre-B I cells. Using peptide sequences obtained from purified p130 we have cloned its gene. The gene encodes a typical cadherin type 1 membrane protein with six extracellular cadherin domains (one pseudo domain) but lacking the catenin-binding site in its cytoplasmic part. Even without this catenin-binding site, p130 mediates Ca(2+)-dependent homotypic adhesion of cells. The interaction of p130 with surrogate light chain is confirmed by co-transfection and co-immunoprecipitation experiments. The expression of p130 is biphasic during the B cell development. Reverse transcriptase-polymerase chain reaction and flow cytometric analyses revealed that it is expressed on B220(+)c-Kit(+) pro-B and pre-B-I cells as well as on B220(+)CD25(-)IgM(+) immature and mature B cells but not on B220(+)CD25(+) pre-B-II cells. It is also expressed in fetal liver, at low levels in myeloid cells, and strongly in intestinal epithelial cells. In the spleen, p130-expressing cells are mainly localized in the marginal zone. We call this B lineage-, intestine-, liver- and leukocyte-expressed gene BILL-cadherin. The possible functions of BILL-cadherin in B cell development are discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Cadherins,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cdh17 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Light Chains,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Light Chains...,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin M,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Variable Region,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Tyrosine Phosphatase...,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
6
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pubmed:volume |
275
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
31134-44
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10906147-Amino Acid Sequence,
pubmed-meshheading:10906147-Animals,
pubmed-meshheading:10906147-Antibodies, Monoclonal,
pubmed-meshheading:10906147-Antigens, CD45,
pubmed-meshheading:10906147-B-Lymphocytes,
pubmed-meshheading:10906147-Base Sequence,
pubmed-meshheading:10906147-Binding Sites,
pubmed-meshheading:10906147-Blotting, Northern,
pubmed-meshheading:10906147-Cadherins,
pubmed-meshheading:10906147-Calcium,
pubmed-meshheading:10906147-Cell Adhesion,
pubmed-meshheading:10906147-Cell Line,
pubmed-meshheading:10906147-Cloning, Molecular,
pubmed-meshheading:10906147-DNA, Complementary,
pubmed-meshheading:10906147-Drosophila,
pubmed-meshheading:10906147-Female,
pubmed-meshheading:10906147-Flow Cytometry,
pubmed-meshheading:10906147-Gene Library,
pubmed-meshheading:10906147-Immunoglobulin Light Chains,
pubmed-meshheading:10906147-Immunoglobulin Light Chains, Surrogate,
pubmed-meshheading:10906147-Immunoglobulin M,
pubmed-meshheading:10906147-Immunoglobulin Variable Region,
pubmed-meshheading:10906147-Intestines,
pubmed-meshheading:10906147-Leukocytes,
pubmed-meshheading:10906147-Liver,
pubmed-meshheading:10906147-Membrane Glycoproteins,
pubmed-meshheading:10906147-Mice,
pubmed-meshheading:10906147-Mice, Inbred BALB C,
pubmed-meshheading:10906147-Mice, Inbred C57BL,
pubmed-meshheading:10906147-Molecular Sequence Data,
pubmed-meshheading:10906147-Precipitin Tests,
pubmed-meshheading:10906147-Protein Structure, Tertiary,
pubmed-meshheading:10906147-Protein Tyrosine Phosphatase, Non-Receptor Type 1,
pubmed-meshheading:10906147-Proto-Oncogene Proteins c-kit,
pubmed-meshheading:10906147-Rats,
pubmed-meshheading:10906147-Receptors, Interleukin-2,
pubmed-meshheading:10906147-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10906147-Time Factors,
pubmed-meshheading:10906147-Tissue Distribution,
pubmed-meshheading:10906147-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain.
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pubmed:affiliation |
Basel Institute for Immunology, Grenzacherstrasse 487, CH-4005 Basel, Switzerland and the Department of Immunology, The Tokyo Metropolitan Institute of Medical Science, Tokyo 113-8613, Japan. ohnishik@nih.go.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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