Source:http://linkedlifedata.com/resource/pubmed/id/10906052
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-10-19
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| pubmed:abstractText |
Formation of mature spermatozoa involves a series of dramatic molecular and morphological changes in the male germ cell lineage. These changes result from the temporally regulated transcription and translation of several testis-specific gene products. Here, we describe a novel, testis-specific protein designated SPAN-X for sperm protein associated with the nucleus on the X chromosome. SPAN-X sequences showed no significant similarity with known cDNA or peptide sequences. The SPAN-X peptide sequences contained three overlapping consensus nuclear localization signals, a high percentage (33%-37%) of charged amino acid residues, and a relatively acidic isoelectric point (pI; 4.88-6.05). Northern analysis of mRNA from multiple human tissues identified a SPAN-X transcript exclusively in the testis. In situ hybridization of human testes sections showed SPAN-X mRNA expression in haploid, round, and elongating spermatids. The SPANX gene was mapped to chromosome Xq27. 1 by fluorescence in situ hybridization and by Southern blot analysis of human/mouse somatic cell hybrids. On Western blots of human sperm proteins, antirecombinant SPAN-X antibodies reacted with broad bands migrating between 15-20 kDa. Immunofluorescent labeling of human spermatozoa demonstrated SPAN-X localization to nuclear craters and cytoplasmic droplets. Expression of SPAN-X, an X-linked gene product, exclusively in haploid spermatids leads to interesting questions regarding the transcription of sex-linked genes during spermiogenesis.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
|
| pubmed:issn |
0006-3363
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| pubmed:author |
pubmed-author:DiekmanA BAB,
pubmed-author:FlickingerC JCJ,
pubmed-author:GoldenW LWL,
pubmed-author:HILDSS,
pubmed-author:HerrJ CJC,
pubmed-author:KholeV VVV,
pubmed-author:KlotzK LKL,
pubmed-author:LeeC YCY,
pubmed-author:ShowsT BTB,
pubmed-author:StolerM HMH,
pubmed-author:WestbrookV AVA,
pubmed-author:von Kap-HerrCC
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| pubmed:issnType |
Print
|
| pubmed:volume |
63
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
469-81
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:10906052-Amino Acid Sequence,
pubmed-meshheading:10906052-Base Sequence,
pubmed-meshheading:10906052-Blotting, Northern,
pubmed-meshheading:10906052-Cell Nucleus,
pubmed-meshheading:10906052-Chromosome Mapping,
pubmed-meshheading:10906052-Genetic Linkage,
pubmed-meshheading:10906052-Haploidy,
pubmed-meshheading:10906052-Humans,
pubmed-meshheading:10906052-In Situ Hybridization,
pubmed-meshheading:10906052-Isoelectric Point,
pubmed-meshheading:10906052-Male,
pubmed-meshheading:10906052-Meiosis,
pubmed-meshheading:10906052-Molecular Sequence Data,
pubmed-meshheading:10906052-Nuclear Proteins,
pubmed-meshheading:10906052-RNA, Messenger,
pubmed-meshheading:10906052-Recombinant Proteins,
pubmed-meshheading:10906052-Spermatids,
pubmed-meshheading:10906052-Testis,
pubmed-meshheading:10906052-Transcription, Genetic,
pubmed-meshheading:10906052-X Chromosome
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| pubmed:year |
2000
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| pubmed:articleTitle |
Spermatid-specific expression of the novel X-linked gene product SPAN-X localized to the nucleus of human spermatozoa.
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| pubmed:affiliation |
Departments of Cell Biology, University of Virginia, Charlottesville, Virginia 22908, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|