Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-9-13
pubmed:abstractText
1. The contractile effects of endothelin-1, isoprenaline and extracellular calcium were assessed on ventricular cardiomyocytes isolated from lipopolysaccharide-treated rats. The involvement of nitric oxide was investigated using dexamethasone (in vivo) and ethyl isothiourea (in vitro). 2. Male Wistar rats (n=70) were injected with either saline (1 ml kg(-1)) or lipopolysaccharide (LPS; 5 mg kg(-1)) alone, or following pre-treatment with dexamethasone (DEX+LPS; 5 mg kg(-1)). Ventricular cell shortening was recorded using a video edge detection system, and concentration-response relationships were established for endothelin-1, isoprenaline and calcium, in the absence or presence of ethyl isothiourea (ETU; 10 microM). iNOS expression was assessed using reverse transcription-polymerase chain reaction. 3. iNOS mRNA expression was greater (P<0.001) in the LPS (iNOS/GAPDH ratio: 0.90+/-0.09) treated group compared to saline (iNOS/GAPDH ratio: 0.36+/-0.02). Baseline contractile amplitude was reduced (P<0.05) in the LPS (7.3+/-0.2 microm) and DEX+LPS groups (6.7+/-0.3 microm) compared to saline (8. 0+/-0.2 microm). 4. The concentration-dependent contractile response to endothelin-1 was attenuated (P<0.05) in the LPS group compared to saline (maximum change: 0.45+/-0.2 vs 1.8+/-0.2 microm). Neither ETU nor dexamethasone improved contractile function in the LPS-treated animals. 5. The concentration-dependent increase in the contractile response to isoprenaline was attenuated in the LPS-treated group compared to saline (P<0.05; maximum change: 1.7+/-0.4 vs 3.1+/-0.4 microm). This effect was reversed by ETU (maximum change: 3.7+/-0.6 microm). Pre-treatment with dexamethasone prevented a significant fall in contraction amplitude (maximum change: 2.4+/-0.4 microm). 6. The contractile response to calcium was reduced (P<0.05) in the LPS group compared to saline (maximum change: 8.7+/-0.6 vs 10.7+/-0.8 microm). Neither ETU nor dexamethasone restored contractile function in the LPS-treated group. 7. In conclusion, a nitric oxide-mediated inhibitory pathway is not responsible for the diminished contractile response to either endothelin-1 or extracellular calcium, but contributes to the hyporesponsiveness to isoprenaline in lipopolysaccharide treated rats.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-10086391, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1481919, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1631560, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1700905, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1702214, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1718778, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1872494, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1998407, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2168817, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2549546, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-3279822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-6703540, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7531606, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7533622, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7539082, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7541961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7769801, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8147551, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8227345, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8486792, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8528549, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8562430, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8564555, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8595520, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8696980, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8997325, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9049599, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9142036, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9165675, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9290578, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9400382, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9405166, http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9511084
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone, http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1, http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins, http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol, http://linkedlifedata.com/resource/pubmed/chemical/Isothiuronium, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II, http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/etiron
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
130
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1275-82
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10903966-Animals, pubmed-meshheading:10903966-Calcium, pubmed-meshheading:10903966-Dexamethasone, pubmed-meshheading:10903966-Dose-Response Relationship, Drug, pubmed-meshheading:10903966-Endothelin-1, pubmed-meshheading:10903966-Endotoxins, pubmed-meshheading:10903966-Heart Ventricles, pubmed-meshheading:10903966-Isoproterenol, pubmed-meshheading:10903966-Isothiuronium, pubmed-meshheading:10903966-Lipopolysaccharides, pubmed-meshheading:10903966-Male, pubmed-meshheading:10903966-Myocardial Contraction, pubmed-meshheading:10903966-Nitric Oxide Synthase, pubmed-meshheading:10903966-Nitric Oxide Synthase Type II, pubmed-meshheading:10903966-RNA, Messenger, pubmed-meshheading:10903966-Rats, pubmed-meshheading:10903966-Rats, Wistar, pubmed-meshheading:10903966-Ventricular Function
pubmed:year
2000
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