rdf:type |
|
lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0011777,
umls-concept:C0014264,
umls-concept:C0022245,
umls-concept:C0034693,
umls-concept:C0079281,
umls-concept:C0205409,
umls-concept:C0225828,
umls-concept:C0871261,
umls-concept:C1280500,
umls-concept:C1522326,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
|
pubmed:issue |
6
|
pubmed:dateCreated |
2000-9-13
|
pubmed:abstractText |
1. The contractile effects of endothelin-1, isoprenaline and extracellular calcium were assessed on ventricular cardiomyocytes isolated from lipopolysaccharide-treated rats. The involvement of nitric oxide was investigated using dexamethasone (in vivo) and ethyl isothiourea (in vitro). 2. Male Wistar rats (n=70) were injected with either saline (1 ml kg(-1)) or lipopolysaccharide (LPS; 5 mg kg(-1)) alone, or following pre-treatment with dexamethasone (DEX+LPS; 5 mg kg(-1)). Ventricular cell shortening was recorded using a video edge detection system, and concentration-response relationships were established for endothelin-1, isoprenaline and calcium, in the absence or presence of ethyl isothiourea (ETU; 10 microM). iNOS expression was assessed using reverse transcription-polymerase chain reaction. 3. iNOS mRNA expression was greater (P<0.001) in the LPS (iNOS/GAPDH ratio: 0.90+/-0.09) treated group compared to saline (iNOS/GAPDH ratio: 0.36+/-0.02). Baseline contractile amplitude was reduced (P<0.05) in the LPS (7.3+/-0.2 microm) and DEX+LPS groups (6.7+/-0.3 microm) compared to saline (8. 0+/-0.2 microm). 4. The concentration-dependent contractile response to endothelin-1 was attenuated (P<0.05) in the LPS group compared to saline (maximum change: 0.45+/-0.2 vs 1.8+/-0.2 microm). Neither ETU nor dexamethasone improved contractile function in the LPS-treated animals. 5. The concentration-dependent increase in the contractile response to isoprenaline was attenuated in the LPS-treated group compared to saline (P<0.05; maximum change: 1.7+/-0.4 vs 3.1+/-0.4 microm). This effect was reversed by ETU (maximum change: 3.7+/-0.6 microm). Pre-treatment with dexamethasone prevented a significant fall in contraction amplitude (maximum change: 2.4+/-0.4 microm). 6. The contractile response to calcium was reduced (P<0.05) in the LPS group compared to saline (maximum change: 8.7+/-0.6 vs 10.7+/-0.8 microm). Neither ETU nor dexamethasone restored contractile function in the LPS-treated group. 7. In conclusion, a nitric oxide-mediated inhibitory pathway is not responsible for the diminished contractile response to either endothelin-1 or extracellular calcium, but contributes to the hyporesponsiveness to isoprenaline in lipopolysaccharide treated rats.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-10086391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1481919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1631560,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1700905,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1702214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1718778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1872494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-1998407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2168817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-2549546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-3279822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-6703540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7531606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7533622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7539082,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7541961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-7769801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8147551,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8227345,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8486792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8528549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8562430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8564555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8595520,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8696980,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-8997325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9049599,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9142036,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9165675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9290578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9400382,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9405166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10903966-9511084
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Dexamethasone,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Endotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Isoproterenol,
http://linkedlifedata.com/resource/pubmed/chemical/Isothiuronium,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/etiron
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0007-1188
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
130
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1275-82
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10903966-Animals,
pubmed-meshheading:10903966-Calcium,
pubmed-meshheading:10903966-Dexamethasone,
pubmed-meshheading:10903966-Dose-Response Relationship, Drug,
pubmed-meshheading:10903966-Endothelin-1,
pubmed-meshheading:10903966-Endotoxins,
pubmed-meshheading:10903966-Heart Ventricles,
pubmed-meshheading:10903966-Isoproterenol,
pubmed-meshheading:10903966-Isothiuronium,
pubmed-meshheading:10903966-Lipopolysaccharides,
pubmed-meshheading:10903966-Male,
pubmed-meshheading:10903966-Myocardial Contraction,
pubmed-meshheading:10903966-Nitric Oxide Synthase,
pubmed-meshheading:10903966-Nitric Oxide Synthase Type II,
pubmed-meshheading:10903966-RNA, Messenger,
pubmed-meshheading:10903966-Rats,
pubmed-meshheading:10903966-Rats, Wistar,
pubmed-meshheading:10903966-Ventricular Function
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pubmed:year |
2000
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