Source:http://linkedlifedata.com/resource/pubmed/id/10899965
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2000-8-17
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pubmed:abstractText |
Phospholipase D-mediated hydrolysis of N-acylethanolamine phospholipids (NAPEs) releases anandamide and other N-acylethanolamines, resulting in different actions at cellular targets in the CNS. Recently, we have demonstrated that these N-acyl lipids accumulate in cultured neocortical neurons subjected to sodium azide-induced cell injury. We here extend the information on the NAPE response, reporting on the composition of N-acylspecies of NAPE, employing a new methodological approach of HPLC-coupled electrospray ionization mass spectrometry. Exposure to sodium azide (5 mM) increased the total amount of NAPE threefold over control levels; however, no alteration of the relative composition of NAPE species was detected. The anandamide precursor (20 : 4-NAPE) constituted only 0.1% of all NAPEs detected in the neurons. Total NAPE species in control cells amounted to 956-1,060 pmol/10(7) cells. Moreover, we detected the presence of an unknown NAPE species with molecular weight identical to 20 : 4-NAPE. This may suggest the presence of a putative stereoisomer of the anandamide precursor with at least one trans-configured double bond in the N-arachidonoyl moiety. These results show that with the present method, neuronal NAPE species can be identified and quantified with respect to N-acyl composition, including a trans-isomer of the anandamide precursor. The anandamide precursor is up-regulated to the same extent as other NAPEs upon neuronal injury.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Plasmalogens,
http://linkedlifedata.com/resource/pubmed/chemical/Polyunsaturated Alkamides,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Azide,
http://linkedlifedata.com/resource/pubmed/chemical/anandamide
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0022-3042
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
861-71
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10899965-Animals,
pubmed-meshheading:10899965-Arachidonic Acids,
pubmed-meshheading:10899965-Cells, Cultured,
pubmed-meshheading:10899965-Chromatography, High Pressure Liquid,
pubmed-meshheading:10899965-Embryo, Mammalian,
pubmed-meshheading:10899965-Mice,
pubmed-meshheading:10899965-Mice, Inbred Strains,
pubmed-meshheading:10899965-Neocortex,
pubmed-meshheading:10899965-Neurons,
pubmed-meshheading:10899965-Phosphatidylethanolamines,
pubmed-meshheading:10899965-Phospholipase D,
pubmed-meshheading:10899965-Phospholipids,
pubmed-meshheading:10899965-Plasmalogens,
pubmed-meshheading:10899965-Polyunsaturated Alkamides,
pubmed-meshheading:10899965-Sodium Azide,
pubmed-meshheading:10899965-Spectrometry, Mass, Secondary Ion
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pubmed:year |
2000
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pubmed:articleTitle |
Determination of the phospholipid precursor of anandamide and other N-acylethanolamine phospholipids before and after sodium azide-induced toxicity in cultured neocortical neurons.
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pubmed:affiliation |
Department of Pharmacology, Royal Danish School of Pharmacy, Copenhagen, Denmark. hsh@mail.dfh.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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