Linked Life Data

10899162

Source:http://linkedlifedata.com/resource/pubmed/id/10899162

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
39
pubmed:dateCreated
2000-10-27
pubmed:databankReference
pubmed:abstractText
The algae of the family Chlamydomonadaceae lack the gene cox3 that encodes subunit III of cytochrome c oxidase in their mitochondrial genomes. This observation has raised the question of whether this subunit is present in cytochrome c oxidase or whether the corresponding gene is located in the nucleus. Cytochrome c oxidase was isolated from the colorless chlamydomonad Polytomella spp., and the existence of subunit III was established by immunoblotting analysis with an antibody directed against Saccharomyces cerevisiae subunit III. Based partly upon the N-terminal sequence of this subunit, oligodeoxynucleotides were designed and used for polymerase chain reaction amplification, and the resulting product was used to screen a cDNA library of Chlamydomonas reinhardtii. The complete sequences of the cox3 cDNAs from Polytomella spp. and C. reinhardtii are reported. Evidence is provided that the genes for cox3 are encoded by nuclear DNA, and the predicted polypeptides exhibit diminished physical constraints for import as compared with mitochondrial-DNA encoded homologs. This indicates that transfer of this gene to the nucleus occurred before Polytomella diverged from the photosynthetic Chlamydomonas lineage and that this transfer may have occurred in all chlamydomonad algae.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
29
pubmed:volume
275
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
30144-52
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10899162-Amino Acid Sequence, pubmed-meshheading:10899162-Angiosperms, pubmed-meshheading:10899162-Animals, pubmed-meshheading:10899162-Cell Nucleus, pubmed-meshheading:10899162-Chlorophyta, pubmed-meshheading:10899162-Electron Transport Complex IV, pubmed-meshheading:10899162-Eukaryota, pubmed-meshheading:10899162-Membrane Proteins, pubmed-meshheading:10899162-Mitochondria, pubmed-meshheading:10899162-Molecular Sequence Data, pubmed-meshheading:10899162-Phylogeny, pubmed-meshheading:10899162-Polymerase Chain Reaction, pubmed-meshheading:10899162-Protein Sorting Signals, pubmed-meshheading:10899162-Protein Structure, Quaternary, pubmed-meshheading:10899162-Saccharomyces cerevisiae Proteins, pubmed-meshheading:10899162-Sequence Analysis, DNA, pubmed-meshheading:10899162-Sequence Homology, Amino Acid
pubmed:year
2000
pubmed:articleTitle
Unusual location of a mitochondrial gene. Subunit III of cytochrome C oxidase is encoded in the nucleus of Chlamydomonad algae.
pubmed:affiliation
Departamento de Genética Molecular, Instituto de Fisiologia Celular, Universidad Nacional Autónoma de México, 04510.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't