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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-7-28
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pubmed:abstractText |
The role of phosphodiesterase type 5 inhibition in the modulation of female sexual dysfunction was investigated by assessing its effects on in vitro relaxation of rabbit clitoris. Stimulation of the non-adrenergic, non-cholinergic neurons of the clitorus elicited a frequency-dependent relaxation response. Inhibition of NO synthase with L-NAME (100 microM) or inhibition of soluble guanylate cyclase with ODQ (1.0 microM) almost completely abolished the electrical field stimulation-induced relaxation of clitorus suggesting that NO-cGMP pathway mediates the relaxation response to electrical field stimulation. Similarly, tetrodotoxin, a neuronal sodium channel blocker abolished the electrical field stimulation-induced clitoral relaxation implying a neuronal release of NO contributes to the electrical field stimulation elicited relaxation. Pretreatment of the clitoral corpus cavernosum strips with sildenafil (100 nM) enhanced the electrical field stimulation-induced relaxations both in magnitude and duration. The results suggest that sildenafil enhances electrical field stimulation elicited clitoral relaxation by a NO-cGMP dependent pathway. These data also imply that sildenafil may be useful to treat female sexual dysfunction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1H-(1,2,4)oxadiazolo(4,3-a)quinoxali...,
http://linkedlifedata.com/resource/pubmed/chemical/3',5'-Cyclic-GMP Phosphodiesterases,
http://linkedlifedata.com/resource/pubmed/chemical/Guanylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Oxadiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphodiesterase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/Purines,
http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfones,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrodotoxin,
http://linkedlifedata.com/resource/pubmed/chemical/sildenafil
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0024-3205
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
26
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10896025-3',5'-Cyclic-GMP Phosphodiesterases,
pubmed-meshheading:10896025-Animals,
pubmed-meshheading:10896025-Clitoris,
pubmed-meshheading:10896025-Electric Stimulation,
pubmed-meshheading:10896025-Female,
pubmed-meshheading:10896025-Guanylate Cyclase,
pubmed-meshheading:10896025-Muscle, Smooth,
pubmed-meshheading:10896025-Muscle Relaxation,
pubmed-meshheading:10896025-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:10896025-Nitric Oxide,
pubmed-meshheading:10896025-Nitric Oxide Synthase,
pubmed-meshheading:10896025-Oxadiazoles,
pubmed-meshheading:10896025-Phosphodiesterase Inhibitors,
pubmed-meshheading:10896025-Piperazines,
pubmed-meshheading:10896025-Purines,
pubmed-meshheading:10896025-Quinoxalines,
pubmed-meshheading:10896025-Rabbits,
pubmed-meshheading:10896025-Sulfones,
pubmed-meshheading:10896025-Synaptic Transmission,
pubmed-meshheading:10896025-Tetrodotoxin
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pubmed:year |
2000
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pubmed:articleTitle |
Sildenafil relaxes rabbit clitoral corpus cavernosum.
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pubmed:affiliation |
CNS/CV Biological Research, Schering-Plough Research Institute, Kenilworth, NJ 07033, USA. Subbarao.Vemulapalli@SPCorp.com
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pubmed:publicationType |
Journal Article
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