Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
15
pubmed:dateCreated
2000-8-17
pubmed:databankReference
pubmed:abstractText
The technique of mRNA differential display was used to identify simultaneously two metabolic genes involved in the degradation of cyclohexanone in a new halotolerant Brevibacterium environmental isolate. In a strategy based only on the knowledge that cyclohexanone oxidation was inducible in this strain, the mRNA population of cells exposed to cyclohexanone was compared to that of control cells using reverse transcription-PCR reactions primed with a collection of 81 arbitrary oligonucleotides. Three DNA fragments encoding segments of flavin monooxygenases were isolated with this technique, leading to the identification of the genes of two distinct cyclohexanone monooxygenases, the enzymes responsible for the oxidation of cyclohexanone. Each monooxygenase was expressed in Escherichia coli and characterized. This work validates the application of mRNA differential display for the discovery of new microbial metabolic genes.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-10222241, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-10397340, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-10467180, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-10510230, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-10543838, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-1261, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-1261545, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-1354393, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-1383934, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-16346796, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-16347162, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-1979162, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-2540966, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-3338974, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-4312, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-4313, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-4349113, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-5119767, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-7507252, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-7683181, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8000547, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8125318, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8341601, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8349564, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8668539, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8703059, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8779579, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-8866478, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9081299, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9538688, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9663681, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9733676, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9758787, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9895269, http://linkedlifedata.com/resource/pubmed/commentcorrection/10894733-9915494
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
182
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4241-8
pubmed:dateRevised
2010-9-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Simultaneous identification of two cyclohexanone oxidation genes from an environmental Brevibacterium isolate using mRNA differential display.
pubmed:affiliation
E. I. DuPont de Nemours Co., Central Research and Development, Wilmington, Delaware 19800-0328, USA.
pubmed:publicationType
Journal Article