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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2000-7-31
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pubmed:abstractText |
Granzyme B (GzmB) is a component of cytotoxic lymphocyte granules that can rapidly initiate apoptosis in target cells. While several procaspases are cleaved and activated by GzmB, the absolute requirement of caspase activation for GzmB-induced apoptosis is controversial. In this report, we demonstrate that GzmB can initiate apoptosis in the absence of caspase-3 activity by directly cleaving DFF45/ICAD to liberate activated DFF40/CAD. DFF45/ICAD cleavage occurs less efficiently in cells that lack caspase-3 activity, suggesting that the caspases normally amplify the GzmB death signal. DFF45/ICAD-deficient mouse embryo fibroblasts are partially resistant to GzmB-induced death, demonstrating the biological importance of DFF45/ICAD for GzmB-mediated apoptosis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apoptosis Regulatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CASP3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Casp3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA fragmentation factor, human,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/GZMB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Granzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Gzmb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/caspase-activated DNase inhibitor
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
621-32
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10894162-Animals,
pubmed-meshheading:10894162-Apoptosis,
pubmed-meshheading:10894162-Apoptosis Regulatory Proteins,
pubmed-meshheading:10894162-Caspase 3,
pubmed-meshheading:10894162-Caspases,
pubmed-meshheading:10894162-Cell Line,
pubmed-meshheading:10894162-Cytotoxicity, Immunologic,
pubmed-meshheading:10894162-DNA Fragmentation,
pubmed-meshheading:10894162-Deoxyribonucleases,
pubmed-meshheading:10894162-Embryo, Mammalian,
pubmed-meshheading:10894162-Enzyme Inhibitors,
pubmed-meshheading:10894162-Fibroblasts,
pubmed-meshheading:10894162-Granzymes,
pubmed-meshheading:10894162-Immunity, Innate,
pubmed-meshheading:10894162-Killer Cells, Lymphokine-Activated,
pubmed-meshheading:10894162-Mice,
pubmed-meshheading:10894162-Protein Processing, Post-Translational,
pubmed-meshheading:10894162-Proteins,
pubmed-meshheading:10894162-Recombinant Proteins,
pubmed-meshheading:10894162-Serine Endopeptidases,
pubmed-meshheading:10894162-Substrate Specificity,
pubmed-meshheading:10894162-T-Lymphocytes, Cytotoxic
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pubmed:year |
2000
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pubmed:articleTitle |
DFF45/ICAD can be directly processed by granzyme B during the induction of apoptosis.
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pubmed:affiliation |
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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