| pubmed-article:10893323 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0026844 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C1135918 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0079395 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0070099 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0018270 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0237477 | lld:lifeskim |
| pubmed-article:10893323 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:10893323 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:10893323 | pubmed:dateCreated | 2000-8-8 | lld:pubmed |
| pubmed-article:10893323 | pubmed:abstractText | In this study, we investigated the mechanisms responsible for the growth-inhibitory action of parathyroid hormone-related protein (PTHRP) in A10 vascular smooth muscle cells (VSMC). Fluorescence-activated cell sorting analysis of serum-stimulated VSMC treated with PTHRP or dibutyryl-cAMP (DBcAMP) demonstrated an enrichment of cells in G1 and a reduction in the S phase. Measurement of DNA synthesis in platelet-derived growth factor-stimulated VSMC treated with DBcAMP revealed that cells became refractory to growth inhibition by 12-16 h, consistent with blockade in mid-G1. cAMP treatment blunted the serum-induced rise in cyclin D1 during cell cycle progression without altering levels of the cyclin-dependent kinase cdk4 or cyclin E and its associated kinase, cdk2. Exposure of cells to PTHRP or cAMP resulted in a reduction in retinoblastoma gene product (Rb) phosphorylation. Immunoblotting of extracts from cAMP-treated cells with antibodies to cdk inhibitors revealed a striking increase in p27(kip1) abundance coincident with the G1 block. Immunoprecipitation with an anti-cyclin D1 antibody of cell lysates prepared from cAMP-treated cells followed by immunoblotting with antisera to p27(kip1) disclosed a threefold increase in p27(kip1) associated with cyclin D1 compared with lysates treated with serum alone. We conclude that PTHRP, by increasing intracellular cAMP, induces VSMC cycle arrest in mid-G1. This occurs secondary to a suppression in cyclin D1 and induction of p27(kip1) expression, which in turn inhibits Rb phosphorylation. | lld:pubmed |
| pubmed-article:10893323 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10893323 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10893323 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10893323 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:10893323 | pubmed:issn | 0193-1849 | lld:pubmed |
| pubmed-article:10893323 | pubmed:author | pubmed-author:MaedaSS | lld:pubmed |
| pubmed-article:10893323 | pubmed:author | pubmed-author:ClemensT LTL | lld:pubmed |
| pubmed-article:10893323 | pubmed:author | pubmed-author:StuartW DWD | lld:pubmed |
| pubmed-article:10893323 | pubmed:author | pubmed-author:FabioG PGP | lld:pubmed |
| pubmed-article:10893323 | pubmed:author | pubmed-author:KheraPP | lld:pubmed |
| pubmed-article:10893323 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10893323 | pubmed:volume | 279 | lld:pubmed |
| pubmed-article:10893323 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10893323 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10893323 | pubmed:pagination | E60-7 | lld:pubmed |
| pubmed-article:10893323 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:10893323 | pubmed:meshHeading | pubmed-meshheading:10893323... | lld:pubmed |
| pubmed-article:10893323 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10893323 | pubmed:articleTitle | Parathyroid hormone-related protein induces G1 phase growth arrest of vascular smooth muscle cells. | lld:pubmed |
| pubmed-article:10893323 | pubmed:affiliation | Departments of Medicine and Molecular and Cellular Physiology, University of Cincinnati, Cincinnati, Ohio 45267, USA. | lld:pubmed |
| pubmed-article:10893323 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10893323 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10893323 | lld:pubmed |
