Source:http://linkedlifedata.com/resource/pubmed/id/10891567
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-9-27
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| pubmed:abstractText |
E-cadherin is a transmembrane glycoprotein which mediates a calcium dependent homophilic interaction among epithelial cells. The altered expression and gene mutations of E-cadherin adhesion molecule have been frequently observed in various tumors. Several invasive carcinomas showed cell-cell adhesion loss although the tumor cells expressed considerable amounts of E-cadherin protein. The purpose of this study was to evaluate the role of E-cadherin gene alterations in genesis and progression of bladder carcinoma by mutation analysis of coding region, expression analysis and microsatellite instability at E-cadherin chromosome locus. We analyzed 30 bladder carcinoma (28 transitional and 2 squamous cell carcinoma) at different stage and grade. The mutation analysis showed that in one case there was a presence of a point mutation at codon 846 that consisted of a G (AGC) to C (ACC) transversion resulting in the replacement of R to T. In another sample the sequence analysis revealed a same-sense mutation at the codon 785 (AAC - AAT). The study of E-cadherin mRNA by Northern blot analysis showed that there were no differences of mRNA levels between tumor and normal mucosa samples. We noted that invasive and anaplastic tumors showed a trend to loss of expression, even if we did not find any statistically significant differences. The microsatellite analysis showed the presence of genomic instability in proximity of the E-cadherin gene. Nine out of 30 (30%) specimens presented molecular alterations in at least one out of 2 loci (D16S260 and D16S301) analyzed. The comparison between microsatellite mutations and clinical-histopathological parameters revealed a higher number of alterations in invasive respect to superficial tumors (p=0.014). On the other hand, there were no statistical differences regarding the correlation with pathological grade. These observations, which, nevertheless, need to be confirmed in a larger number of patients, suggest that alterations of E-cadherin gene may be related to pathobiology of bladder cancer development and clinical progression.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
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| pubmed:issn |
1107-3756
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
6
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
201-8
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10891567-Aged,
pubmed-meshheading:10891567-Aged, 80 and over,
pubmed-meshheading:10891567-Amino Acid Substitution,
pubmed-meshheading:10891567-Cadherins,
pubmed-meshheading:10891567-Carcinoma, Squamous Cell,
pubmed-meshheading:10891567-Carcinoma, Transitional Cell,
pubmed-meshheading:10891567-DNA Mutational Analysis,
pubmed-meshheading:10891567-Female,
pubmed-meshheading:10891567-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10891567-Humans,
pubmed-meshheading:10891567-Loss of Heterozygosity,
pubmed-meshheading:10891567-Male,
pubmed-meshheading:10891567-Microsatellite Repeats,
pubmed-meshheading:10891567-Middle Aged,
pubmed-meshheading:10891567-Neoplasm Staging,
pubmed-meshheading:10891567-Polymorphism, Single-Stranded Conformational,
pubmed-meshheading:10891567-RNA, Messenger,
pubmed-meshheading:10891567-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10891567-Urinary Bladder Neoplasms
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| pubmed:year |
2000
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| pubmed:articleTitle |
Molecular alterations of E-cadherin gene: possible role in human bladder carcinogenesis.
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| pubmed:affiliation |
Medical Genetics Unit, Department of Clinical Physiopathology, Florence University Medical School, Florence, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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