Source:http://linkedlifedata.com/resource/pubmed/id/10891440
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2000-8-10
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| pubmed:abstractText |
To investigate the tropism of the T-lymphotropic human herpesvirus 7 (HHV-7) for hematopoietic progenitors, cord blood CD34(+) cells were inoculated in vitro with HHV-7 and then induced to differentiate along the granulocytic and erythroid lineages by the addition of appropriate cytokine cocktails. In semisolid assays, HHV-7 modestly affected the growth of committed (granulocytic/macrophagic and erythroid) progenitors, whereas it significantly decreased the number of pluripotent (granulocytic/erythroid/ monocytic/megakaryocytic) progenitors. Such inhibitory effect was completely abrogated by incubating HHV-7 inoculum with anti-HHV-7 neutralizing serum. In liquid cultures, HHV-7 hastened maturation along the myeloid but not the erythroid lineage, as demonstrated by the up-regulation of CD33 early myeloid antigen at day 7 of culture, and of CD15 and CD14 antigens at day 15. Moreover, HHV-7 messenger RNA was detected by reverse transcriptase-polymerase chain reaction (RT-PCR) in cells maturating along both the myeloid and the erythroid lineages. To evaluate the relevance of these in vitro findings, the presence of HHV-7 was investigated in bone marrow (BM) unfractionated mononuclear cells (MCs) as well as in purified CD34(+) and CD34(-) cell subsets, obtained from 14 normal adult donors. HHV-7 DNA was detected by DNA-PCR in 4 of 7 BMMC samples, and it was found to be associated with both the CD34(-) (2 of 7) and the CD34(+ )(1 of 7) fractions. These data indicate that HHV-7 infects BM cells in vivo and shows the ability to affect the survival/differentiation of CD34(+) hematopoietic progenitors in vitro by inhibiting more ancestral progenitors and perturbing the maturation of myeloid cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
|
| pubmed:issn |
0006-4971
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
96
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
126-31
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10891440-Adult,
pubmed-meshheading:10891440-Antigens, CD,
pubmed-meshheading:10891440-Antigens, CD34,
pubmed-meshheading:10891440-Bone Marrow Cells,
pubmed-meshheading:10891440-Cell Differentiation,
pubmed-meshheading:10891440-Cell Line,
pubmed-meshheading:10891440-DNA, Viral,
pubmed-meshheading:10891440-DNA Replication,
pubmed-meshheading:10891440-Erythrocytes,
pubmed-meshheading:10891440-Fetal Blood,
pubmed-meshheading:10891440-Granulocytes,
pubmed-meshheading:10891440-Hematopoiesis,
pubmed-meshheading:10891440-Hematopoietic Stem Cells,
pubmed-meshheading:10891440-Herpesvirus 7, Human,
pubmed-meshheading:10891440-Humans,
pubmed-meshheading:10891440-Infant, Newborn,
pubmed-meshheading:10891440-RNA, Messenger,
pubmed-meshheading:10891440-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10891440-T-Lymphocytes,
pubmed-meshheading:10891440-Virus Replication
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| pubmed:year |
2000
|
| pubmed:articleTitle |
Infection of CD34(+) hematopoietic progenitor cells by human herpesvirus 7 (HHV-7).
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| pubmed:affiliation |
Institute of Normal Morphology, G. D'Annunzio University of Chieti, Chieti, Italy.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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