Linked Life Data

10891409

Source:http://linkedlifedata.com/resource/pubmed/id/10891409

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-11
pubmed:abstractText
To determine whether live-attenuated feline immunodeficiency virus (FIV) proviral DNA will induce protective immunity, a plasmid clone constructed with a FIV provirus containing a deletion in the viral accessory gene vif (FIV-pPPR-Deltavif) was inoculated as proviral DNA into four cats by the intramuscular route. After 43 weeks, these cats were boosted with the same proviral plasmid. Analysis of peripheral blood mononuclear cells at several time points after the primary and booster inoculations revealed no detectable virus or proviral DNA. At 6 weeks after the booster, immunized cats and additional naive control cats were challenged with a cell-free preparation of the infectious biological isolate FIV-PPR by the intraperitoneal route. Virus was detected after challenge in unvaccinated control cats but not in any of the FIV-pPPR-Deltavif-immunized cats. Both FIV Gag- and Env-specific cytotoxic T lymphocyte (CTL) activities were detected in peripheral blood cells of control cats after challenge infection, whereas only one of four cats immunized with FIV-pPPR-Deltavif DNA exhibited a measurable CTL response to Env following challenge. Although anti-Gag antibodies were not detected after both proviral DNA inoculation and challenge, anti-Env antibodies were found in FIV-pPPR-Deltavif-immunized cats after vaccination as well as after challenge. These findings indicate that inoculation with FIV-pPPR-Deltavif proviral DNA induced resistance to challenge with infectious FIV and that a vif deletion mutant may provide a relatively safe attenuated lentiviral vaccine.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0042-6822
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
20
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-79
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10891409-Animals, pubmed-meshheading:10891409-Antibodies, Viral, pubmed-meshheading:10891409-Antigens, Viral, pubmed-meshheading:10891409-Cats, pubmed-meshheading:10891409-DNA, Viral, pubmed-meshheading:10891409-Gene Deletion, pubmed-meshheading:10891409-Gene Products, env, pubmed-meshheading:10891409-Gene Products, gag, pubmed-meshheading:10891409-Gene Products, vif, pubmed-meshheading:10891409-Genome, Viral, pubmed-meshheading:10891409-Immunodeficiency Virus, Feline, pubmed-meshheading:10891409-Lentivirus Infections, pubmed-meshheading:10891409-Lymphocyte Count, pubmed-meshheading:10891409-Proviruses, pubmed-meshheading:10891409-T-Lymphocytes, Cytotoxic, pubmed-meshheading:10891409-Time Factors, pubmed-meshheading:10891409-Vaccines, DNA, pubmed-meshheading:10891409-Viral Vaccines
pubmed:year
2000
pubmed:articleTitle
Protective immunity against feline immunodeficiency virus induced by inoculation with vif-deleted proviral DNA.
pubmed:affiliation
Departments of Medicine and Epidemiology, University of California, Davis, California, 95616, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't