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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-9-8
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pubmed:abstractText |
We have identified a cDNA, designated HOFNH30, which encodes a 354 amino acid G-protein-coupled receptor (GPCR). This receptor has 96% amino acid identity to the Jurkat-T cell-derived EDG7 and could be a splice variant. RT-PCR analysis demonstrated that HOFNH30 mRNA is expressed in placenta whereas EDG7 mRNA shows highest expression in prostate. The HOFNH30 gene is localized to human chromosome 1p22. 3-1p31.1. When HOFNH30 was expressed in RBL-2H3 cells, LPA and phosphatidic acid (PA) induced a calcium mobilization response with EC(50) values of 13 nM and 3 microM, respectively. LPA also induced phosphorylation of mitogen-activated protein kinase (p42(MAPK) and p44(MAPK)) in HOFNH30-transfected but not vector-transfected RBL-2H3 cells. In the present study, we have identified a novel variant from the EDG receptor family, a GPCR for which LPA is a high-affinity endogenous ligand.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lysophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lysophosphatidic Acid
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:AmesR SRS,
pubmed-author:BergsmaD JDJ,
pubmed-author:CampbellD ADA,
pubmed-author:DytkoG MGM,
pubmed-author:EllisCC,
pubmed-author:ElshourbagyN ANA,
pubmed-author:EvansNN,
pubmed-author:FitzgeraldL RLR,
pubmed-author:FoleyJ JJJ,
pubmed-author:LaneP APA,
pubmed-author:MannanI JIJ,
pubmed-author:McMillanLL,
pubmed-author:MinehartHH,
pubmed-author:MurdockP RPR,
pubmed-author:SarauH MHM,
pubmed-author:TanK BKB,
pubmed-author:TsuiPP,
pubmed-author:WilsonSS
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
805-10
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10891327-Amino Acid Sequence,
pubmed-meshheading:10891327-Base Sequence,
pubmed-meshheading:10891327-Calcium,
pubmed-meshheading:10891327-Chromosome Mapping,
pubmed-meshheading:10891327-Chromosomes, Human, Pair 1,
pubmed-meshheading:10891327-Cloning, Molecular,
pubmed-meshheading:10891327-Enzyme Activation,
pubmed-meshheading:10891327-GTP-Binding Proteins,
pubmed-meshheading:10891327-Humans,
pubmed-meshheading:10891327-Jurkat Cells,
pubmed-meshheading:10891327-Lysophospholipids,
pubmed-meshheading:10891327-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:10891327-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:10891327-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10891327-Molecular Sequence Data,
pubmed-meshheading:10891327-Phosphorylation,
pubmed-meshheading:10891327-Receptors, Cell Surface,
pubmed-meshheading:10891327-Receptors, G-Protein-Coupled,
pubmed-meshheading:10891327-Receptors, Lysophosphatidic Acid,
pubmed-meshheading:10891327-Sequence Homology, Amino Acid,
pubmed-meshheading:10891327-Tumor Cells, Cultured
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pubmed:year |
2000
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pubmed:articleTitle |
Identification of an EDG7 variant, HOFNH30, a G-protein-coupled receptor for lysophosphatidic acid.
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pubmed:affiliation |
Department of Renal Biology, Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, 709 Swedeland Road, King of Prussia, Pennsylvania 19406, USA.
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pubmed:publicationType |
Journal Article
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