Linked Life Data

10890723

Source:http://linkedlifedata.com/resource/pubmed/id/10890723

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-7-25
pubmed:abstractText
Interleukin-1alpha (IL-1alpha) and matrix metalloproteinase-9 (MMP-9) are thought to be involved in odontogenic cyst expansion. In this study, we investigated the effects of IL-1alpha on the secretion and activation of MMP-9 in odontogenic jaw cysts. An active form of MMP-9 was present in odontogenic keratocyst (6 of 8 cases) fluids more frequently than dentigerous cyst (3 of 10 cases) and radicular cyst (3 of 10 cases) fluids, although proMMP-9 was present in all cyst fluids. Odontogenic keratocyst fragments in explant culture secreted a larger amount of IL-1alpha than dentigerous cyst and radicular cyst fragments in explant culture, and spontaneously secreted both proMMP-9 and an active form of MMP-9. The fragments of dentigerous cysts and radicular cysts secreted a small amount of proMMP-9, but no active form of MMP-9. Exogenously added recombinant human IL-1alpha (rhlL-1alpha) increased the secretion and activation of proMMP-9 in the fragments of dentigerous cysts and radicular cysts. The epithelial cells isolated from odontogenic keratocysts secreted IL-1alpha and proMMP-9 without stimulation. Under the cultivation on a fibronectin-coated dish, rhIL-1alpha increased the secretion of proMMP-9 from the epithelial cells in a dose-dependent manner. Moreover, rhIL-1alpha induced the secretion of proMMP-3 and plasminogen activator urokinase (u-PA) from the epithelial cells, and converted the secreted proMMP-3 to the active form in the presence of plasminogen. The secreted proMMP-9 was also activated in the presence of rhIL-1alpha and plasminogen. Hence, our results suggest that IL-1alpha may up-regulate not only proMMP-9 secretion but also proMMP-9 activation by inducing proMMP-3 and u-PA production in the cyst epithelial cells by autocrine/paracrine regulatory mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1423-30
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10890723-Autocrine Communication, pubmed-meshheading:10890723-Cells, Cultured, pubmed-meshheading:10890723-Culture Media, pubmed-meshheading:10890723-Cyst Fluid, pubmed-meshheading:10890723-Dentigerous Cyst, pubmed-meshheading:10890723-Dose-Response Relationship, Drug, pubmed-meshheading:10890723-Enzyme Activation, pubmed-meshheading:10890723-Enzyme Precursors, pubmed-meshheading:10890723-Epithelial Cells, pubmed-meshheading:10890723-Fibronectins, pubmed-meshheading:10890723-Humans, pubmed-meshheading:10890723-Interleukin-1, pubmed-meshheading:10890723-Matrix Metalloproteinase 3, pubmed-meshheading:10890723-Matrix Metalloproteinase 9, pubmed-meshheading:10890723-Odontogenic Cysts, pubmed-meshheading:10890723-Paracrine Communication, pubmed-meshheading:10890723-Plasminogen Activators, pubmed-meshheading:10890723-Radicular Cyst, pubmed-meshheading:10890723-Recombinant Proteins, pubmed-meshheading:10890723-Up-Regulation, pubmed-meshheading:10890723-Urokinase-Type Plasminogen Activator
pubmed:year
2000
pubmed:articleTitle
Interleukin-1alpha-dependent regulation of matrix metalloproteinase-9(MMP-9) secretion and activation in the epithelial cells of odontogenic jaw cysts.
pubmed:affiliation
Second Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Kyushu University, Fukuoka, Japan. yasu@dent.kyushu-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't