Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-7-25
pubmed:abstractText
The anti-oxidant enzyme system protects cellular macromolecules against damage from reactive oxygen species. One component of this system, manganese superoxide dismutase (MnSOD), has also been shown to display tumor suppressor gene-like activity. The purpose of this study was to examine changes in MnSOD expression during hamster cheek pouch carcinogenesis, and the effects of MnSOD overexpression using an adenoviral vector. Tumor induction was carried out using 7,12-dimethylbenz[alpha]anthracene. Animals were killed at periodic intervals, and cheek pouch tissues were excised and examined for MnSOD expression by immunohistochemistry and digital image analysis. We observed a reduction in MnSOD expression as early as 2 weeks after the start of carcinogen application. Low MnSOD expression persisted until the end of the 23-week experimental period. Solid hamster cheek pouch carcinoma xenografts were then established in nude mice. An adenoviral vector encoding the human MnSOD gene was delivered to the xenografts by direct injection. We observed high, immediate expression of MnSOD in the xenografts that persisted for 10 days following cessation of viral construct delivery. Delivery of the MnSOD construct resulted in a maximal 50% reduction in tumor growth compared with untreated controls. Our results suggest that MnSOD may be a tumor suppressor gene in the hamster cheek pouch model system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1410-7
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10890721-9,10-Dimethyl-1,2-benzanthracene, pubmed-meshheading:10890721-Adenoviridae, pubmed-meshheading:10890721-Analysis of Variance, pubmed-meshheading:10890721-Animals, pubmed-meshheading:10890721-Anticarcinogenic Agents, pubmed-meshheading:10890721-Antioxidants, pubmed-meshheading:10890721-Carcinogens, pubmed-meshheading:10890721-Cheek, pubmed-meshheading:10890721-Cricetinae, pubmed-meshheading:10890721-Free Radical Scavengers, pubmed-meshheading:10890721-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10890721-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10890721-Gene Transfer Techniques, pubmed-meshheading:10890721-Genes, Tumor Suppressor, pubmed-meshheading:10890721-Genetic Vectors, pubmed-meshheading:10890721-Humans, pubmed-meshheading:10890721-Image Processing, Computer-Assisted, pubmed-meshheading:10890721-Immunohistochemistry, pubmed-meshheading:10890721-Male, pubmed-meshheading:10890721-Mesocricetus, pubmed-meshheading:10890721-Mice, pubmed-meshheading:10890721-Mice, Nude, pubmed-meshheading:10890721-Mouth Neoplasms, pubmed-meshheading:10890721-Neoplasm Transplantation, pubmed-meshheading:10890721-Superoxide Dismutase, pubmed-meshheading:10890721-Transplantation, Heterologous
pubmed:year
2000
pubmed:articleTitle
Immunolocalization and adenoviral vector-mediated manganese superoxide dismutase gene transfer to experimental oral tumors.
pubmed:affiliation
Department of Radiology, University of Iowa College of Medicine, Iowa City, USA. ernest.lam@ualberta.ca
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't