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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-7-18
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pubmed:abstractText |
To determine whether the paracrine secretion of interleukin (IL)-12 can efficiently convert immune responses characterized by high levels of synthesis of IL-4 and immunoglobulin E (IgE) into T helper 1 (Th1)-dominated responses, 3T3 fibroblasts were stably transfected to secrete IL-12 (480 units/10(6) cells/48 hr). Their effects on the T helper cell-mediated immune response were investigated in ovalbumin (OVA)-primed mice. Free mouse recombinant IL-12 was included as a control group. IL-12-secreting fibroblasts (3T3/IL-12) were more effective than free recombinant IL-12 at increasing OVA-specific interferon-gamma (IFN-gamma) production and decreasing OVA-specific IL-4 production in CD4+ T cells. In addition, injection with 3T3/IL-12 cells significantly increased anti-OVA immunoglobulin G2a (IgG2a) levels and decreased anti-OVA IgE levels in OVA-primed mice. This work suggests that IL-12-secreting fibroblasts can efficiently induce an antigen-specific Th1 response and may be beneficial in the treatment of diseases caused by undesirable T helper 2 (Th2)-dominated responses, including allergic diseases.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-10076609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-10447770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-10499605,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-2524278,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-7903204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-7904381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-7909326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-8094711,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/10886396-9762559
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
203-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10886396-Animals,
pubmed-meshheading:10886396-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10886396-Cell Culture Techniques,
pubmed-meshheading:10886396-Dose-Response Relationship, Immunologic,
pubmed-meshheading:10886396-Female,
pubmed-meshheading:10886396-Fibroblasts,
pubmed-meshheading:10886396-Immunization,
pubmed-meshheading:10886396-Immunoglobulin E,
pubmed-meshheading:10886396-Immunoglobulin G,
pubmed-meshheading:10886396-Interferon-gamma,
pubmed-meshheading:10886396-Interleukin-12,
pubmed-meshheading:10886396-Interleukin-4,
pubmed-meshheading:10886396-Mice,
pubmed-meshheading:10886396-Mice, Inbred BALB C,
pubmed-meshheading:10886396-Ovalbumin,
pubmed-meshheading:10886396-Th1 Cells,
pubmed-meshheading:10886396-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Efficient induction of an antigen-specific, T helper type 1 immune response by interleukin-12-secreting fibroblasts.
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pubmed:affiliation |
College of Pharmacy and College of Medicine, Chonnam National University, Kwangju, Republic of Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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