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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-10-12
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pubmed:abstractText |
Apoptosis of inflammatory cells plays a crucial role in the recovery from autoimmune CNS disease. However, the underlying mechanisms of apoptosis induction are as yet ill-defined. Here we report on the neuronal expression of FasL and its potential function in inducing T-cell apoptosis. Using a combination of facial nerve axotomy and passive transfer encephalomyelitis, the fate of CD4+ encephalitogenic T cells engineered to express the gene for green fluorescent protein was followed. FasL gene transcripts and FasL protein were detected in neurons by in sit-hybridization and immunohistochemistry. T cells infiltrating preferentially the injured brain parenchyma were found in the immediate vicinity of FasL expressing neurons and even inside their perikarya. In contrast to neurons, T cells rapidly underwent apoptosis. In co-cultures of hippocampal nerve cells and CD4 T lymphocytes, we confirmed expression of FasL in neurons and concomitant induction of T-cell death. Antibodies blocking neuronal FasL were shown to have a protective effect on T-cell survival. Thus, FasL expression by neurons in neuroinflammatory diseases may constitute a pivotal mechanism underlying apoptosis of encephalitogenic T cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1015-6305
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
353-64
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10885654-Animals,
pubmed-meshheading:10885654-Antibodies,
pubmed-meshheading:10885654-Apoptosis,
pubmed-meshheading:10885654-Brain,
pubmed-meshheading:10885654-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10885654-Cell Death,
pubmed-meshheading:10885654-Coculture Techniques,
pubmed-meshheading:10885654-Encephalomyelitis,
pubmed-meshheading:10885654-Facial Nerve,
pubmed-meshheading:10885654-Fas Ligand Protein,
pubmed-meshheading:10885654-Gene Expression,
pubmed-meshheading:10885654-Green Fluorescent Proteins,
pubmed-meshheading:10885654-Hippocampus,
pubmed-meshheading:10885654-Immunohistochemistry,
pubmed-meshheading:10885654-In Situ Hybridization,
pubmed-meshheading:10885654-Indicators and Reagents,
pubmed-meshheading:10885654-Luminescent Proteins,
pubmed-meshheading:10885654-Membrane Glycoproteins,
pubmed-meshheading:10885654-Motor Neurons,
pubmed-meshheading:10885654-Neurons,
pubmed-meshheading:10885654-RNA, Messenger,
pubmed-meshheading:10885654-Rats,
pubmed-meshheading:10885654-Rats, Inbred Lew
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pubmed:year |
2000
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pubmed:articleTitle |
Neuronal FasL induces cell death of encephalitogenic T lymphocytes.
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pubmed:affiliation |
Department of Neuroimmunology, Max-Planck-Institute of Neurobiology, Martinsried, FRG.
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pubmed:publicationType |
Journal Article
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