Source:http://linkedlifedata.com/resource/pubmed/id/10884344
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2000-8-10
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| pubmed:databankReference | |
| pubmed:abstractText |
In wild-type Escherichia coli, recognition of the recombination hotspot, chi (5'-GCTGGTGG-3'), by the RecBCD enzyme is central to homologous recombination. However, in the recC* class of RecBCD mutants, stimulation of recombination by the canonical chi sequence is not detectable, but the levels of homologous recombination are nearly wild-type. In vivo studies demonstrate that a member of this class of mutants, the recC1004 allele, encodes an enzyme that responds to a novel variant of chi, termed chi* (5'-GCTGGTGCTCG-3'). Here, we establish that, in vitro, the chi* sequence is recognized more efficiently by the RecBC(1004)D enzyme than is the wild-type chi. This is manifest by both a greater modification of nuclease activity and a higher stimulation of RecA protein-mediated joint molecule formation at chi* than at chi. Sequencing of the recC1004 gene revealed that it contains a frameshift mutation, which results in a replacement of nine of the wild-type amino acid residues by eight in the mutant protein, and defines a locus that is important for the specificity of chi-recognition. In addition, we show that this novel, 11 nucleotide chi* sequence also regulates the wild-type RecBCD enzyme, supporting the notion that variants of the canonical chi constitute a class of sequences that regulate the recombination function of RecBCD enzyme.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Exodeoxyribonuclease V,
http://linkedlifedata.com/resource/pubmed/chemical/Exodeoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Rec A Recombinases,
http://linkedlifedata.com/resource/pubmed/chemical/exodeoxyribonuclease V, E coli
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0022-2836
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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| pubmed:issnType |
Print
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| pubmed:day |
14
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| pubmed:volume |
300
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
469-79
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10884344-Alleles,
pubmed-meshheading:10884344-Amino Acid Sequence,
pubmed-meshheading:10884344-Base Pairing,
pubmed-meshheading:10884344-Base Sequence,
pubmed-meshheading:10884344-DNA, Bacterial,
pubmed-meshheading:10884344-DNA, Single-Stranded,
pubmed-meshheading:10884344-Enzyme Activation,
pubmed-meshheading:10884344-Escherichia coli,
pubmed-meshheading:10884344-Escherichia coli Proteins,
pubmed-meshheading:10884344-Exodeoxyribonuclease V,
pubmed-meshheading:10884344-Exodeoxyribonucleases,
pubmed-meshheading:10884344-Genetic Variation,
pubmed-meshheading:10884344-Molecular Sequence Data,
pubmed-meshheading:10884344-Mutation,
pubmed-meshheading:10884344-Nucleotides,
pubmed-meshheading:10884344-Plasmids,
pubmed-meshheading:10884344-Rec A Recombinases,
pubmed-meshheading:10884344-Recombination, Genetic,
pubmed-meshheading:10884344-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:10884344-Substrate Specificity
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| pubmed:year |
2000
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| pubmed:articleTitle |
A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi.
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| pubmed:affiliation |
Sections of Microbiology and of Molecular and Cellular Biology, University of California at Davis, CA 95616, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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