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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-9-2
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pubmed:abstractText |
The cross reactivity of sera from rabbits sensitized to performic acid oxidized ferredoxin (O-Fd) and of spleen cells from mice sensitized to O-Fd was analysed using several chemically modified forms of ferredoxin in the complement fixation test and the in vitro lymphocyte stimulation assay. Only O-Fd and native ferredoxin (native-Fd) gave positive responses in both assays. Dinitrophenylated-O-Fd (DNP-O-Fd) and acid precipitated ferredoxin (TCA-Fd) were able to fix complement (C') but did not simulate DNA synthesis in vitro. Ferredoxin alkylated with N-ethylmaleimioxin (CM-Fd) was unable to stimulate DNA synthesis and was marginally able to fix C'. Methylated-O-Fd (meth-O-Fd) was not recognized in either assay. The various ferredoxin preparations were tested for their ability to sensitize mice for use in the in vitro lymphocyte stimulation assay. Only O-Fd, NEM-Fd and native-Fd were capable of sensitizing lymphocytes for a proliferative response in vitro to the test antigens. This correlates with the observation that only these antigens were able to induce DNA synthesis in O-Fd-sensitized lymphocytes. The nature of the cells responding in vitro was examined by treating the cells with rabbit anti-mouse immunoglobulin and C' or rabbit anti-mouse brain associated theta and C'. The 24-hr response was found to be sensitive to both sera while the 120-hr response was sensitive only to the anti-theta sera.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-1080668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-125723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-13673011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-13873716,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-14236643,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-14435995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4097588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4115688,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4118415,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4138630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4162289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4181247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4399614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4541326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4559167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4566301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4607213,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4652874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4766957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4854219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4881359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4994445,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-4997585,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-5558070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-5722257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-5803788,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-6017286,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1088421-68923
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
31
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
379-88
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1088421-Alkylation,
pubmed-meshheading:1088421-Animals,
pubmed-meshheading:1088421-Complement Fixation Tests,
pubmed-meshheading:1088421-Cross Reactions,
pubmed-meshheading:1088421-Ferredoxins,
pubmed-meshheading:1088421-Immunity, Cellular,
pubmed-meshheading:1088421-Lymphocyte Activation,
pubmed-meshheading:1088421-Mice,
pubmed-meshheading:1088421-Oxidation-Reduction,
pubmed-meshheading:1088421-T-Lymphocytes
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pubmed:year |
1976
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pubmed:articleTitle |
The immune response to oxidized ferredoxin. II. Cross reactivity of cells and antisera to modified ferredoxins and the nature of the cells responding in vitro.
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pubmed:publicationType |
Journal Article,
In Vitro
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