Linked Life Data

10883274

Source:http://linkedlifedata.com/resource/pubmed/id/10883274

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-7-19
pubmed:abstractText
Plasmid pRL-B1 was constructed from detoxifying gene(called B1) of pesticide resistant Culex and from plasmid pRL-439 containing the strong promoter PpsbA. E. coli-cyanobacteria shuttle expression plasmid pDC-B1 was constructed from shuttle vector pDC-8 and from recombinant plasmid pRL-B1, then it was transferred into Synechococcus sp. PCC7942 by triparental conjugative transfer. The existence of B1 was detected by Southern analysis, and the expression of B1 was confirmed by enzyme activity analysis of detoxification of transgenic cyanobacteria. Experimental results indicated that the transgenic cyanobacteria could degrade beta-naphthyl acetate(beta-NA), a specific substrate of esterase. The enzyme activity of transgenic strain was higher than that of the wild type. It may be the first report on transformation of detoxify gene of pesticide resistant culex into Synechococcus strain.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1000-3061
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
42-5
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
[Cloning and expression of the detoxifying gene in cyanobacteria].
pubmed:affiliation
Institute of Genetics, Shandong Agricultural University, Taian.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't