Source:http://linkedlifedata.com/resource/pubmed/id/10880253
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
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| pubmed:dateCreated |
2000-9-8
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| pubmed:abstractText |
The aim of this study was to quantitatively compare expression of mRNA for IL-5 and IFN-gamma with the frequency of mRNA-positive cells, total and activated eosinophils, neutrophils, lymphocytes, and vessels expressing adhesion molecules. Replicate biopsies of skin LPR to pollen antigens (Ag) and control injection sites (B) at 6 and 24 h were assessed for: (1) mRNA for IL-5 and IFN-gamma by quantitative RT-PCR (QC-RT/PCR); (2) frequency of cells expressing mRNA for IL-5 and IFN-gamma by in situ hybridization (ISH); (3) inflammatory cells and adhesion molecule expression. More mRNA for IL-5 was found in Ag- than in B-injected sites at 6 and 24 h by both QC-RT/PCR and ISH. Small amounts of mRNA for IFN-gamma were detected in Ag sites by QC-RT/PCR at 6 and 24 h, but were not significantly different than at B sites. The frequency of IFN-gamma mRNA(+)cells was higher in Ag than in B sites at 6 h. There was no correlation between the amount if IL-5 detected by QC-RT/PCR and frequency of IL-5 mRNA(+)cells by ISH. These findings also did not correlate with the degree of inflammatory responses. In conclusion: (1) greater IL-5 than IFN-gamma deposition in Ag sites suggests Th(2)predominance in LPR; (2) lack of correlation between QC-RT/PCR and ISH findings may reflect varying mRNA content of inflammatory cells.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/E-Selectin,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-5,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1043-4666
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1065-75
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10880253-Adult,
pubmed-meshheading:10880253-Dermatitis,
pubmed-meshheading:10880253-E-Selectin,
pubmed-meshheading:10880253-Eosinophils,
pubmed-meshheading:10880253-Female,
pubmed-meshheading:10880253-Humans,
pubmed-meshheading:10880253-Hypersensitivity,
pubmed-meshheading:10880253-Immunohistochemistry,
pubmed-meshheading:10880253-In Situ Hybridization,
pubmed-meshheading:10880253-Interferon-gamma,
pubmed-meshheading:10880253-Interleukin-5,
pubmed-meshheading:10880253-Leukocytes, Mononuclear,
pubmed-meshheading:10880253-Male,
pubmed-meshheading:10880253-Middle Aged,
pubmed-meshheading:10880253-Neutrophils,
pubmed-meshheading:10880253-Pollen,
pubmed-meshheading:10880253-RNA, Messenger,
pubmed-meshheading:10880253-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10880253-Skin,
pubmed-meshheading:10880253-T-Lymphocytes,
pubmed-meshheading:10880253-Time Factors,
pubmed-meshheading:10880253-Vascular Cell Adhesion Molecule-1
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| pubmed:year |
2000
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| pubmed:articleTitle |
Quantitative comparison of cytokine mRNA and inflammatory responses in cutaneous late phase allergic reactions.
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| pubmed:affiliation |
Department of Dermatology, University of Pennsylvania Medical Center, Philadelphia 19104-6057, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|