Linked Life Data

10880075

Source:http://linkedlifedata.com/resource/pubmed/id/10880075

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-10-19
pubmed:abstractText
Surfaces covered with polyethylene glycol (PEG; HO-(CH(2)-CH(2)-O)(n)-H) have been shown to be biocompatible because PEG's properties yield nonimmunogenicity, nonantigenicity, and protein rejection. To produce a biocompatible surface coating, we have developed a method for grafting PEG onto activated silica films. We first deposited an amorphous silica film by plasma-enhanced chemical vapor deposition from SiH(4) and O(2) gases, which provided the flexibility to coat diverse materials with different chemistries and shapes. The silica films were activated by exposure to water plasma, increasing the number of silanol groups (Si-OH) on their surface. The surface silanol groups were then chemically reacted with the hydroxyl end of PEG to form an ester bond, Si-O-C, and to cover the surface with PEG. The surface reactions were monitored using attenuated total reflection Fourier transform infrared spectroscopy. The vibrational absorption bands of the C-O and -CH(2) bonds increased with time and saturated, indicating that PEG was adsorbed to saturation coverage on the surface. Simultaneously, the Si-OH absorption band decreased, showing that the surface silanols reacted with PEG and were depleted. The PEG-covered surfaces were physically characterized by atomic force microscopy, Auger electron spectroscopy, ellipsometry, and contact angle measurements. These characterization techniques provided additional evidence for the existence of chemically bonded PEG on the surfaces. Efficacy of protein rejection on PEG-covered surfaces was studied through measurements of the fluorescence intensity of Texas red-labeled bovine serum albumin brought in contact with such surfaces in solution. Significantly less protein adsorption was observed on surfaces covered with PEG compared to uncovered surfaces.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0021-9304
pubmed:author
pubmed:copyrightInfo
Copyright 2000 John Wiley & Sons, Inc.
pubmed:issnType
Print
pubmed:day
5
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
343-51
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Polyethylene glycol-coated biocompatible surfaces.
pubmed:affiliation
Chemical Engineering Department and Materials Department, University of California, Santa Barbara, California 93106, USA.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't