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rdf:type |
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lifeskim:mentions |
umls-concept:C0014836,
umls-concept:C0017262,
umls-concept:C0026809,
umls-concept:C0039194,
umls-concept:C0185117,
umls-concept:C0205263,
umls-concept:C0439097,
umls-concept:C0754728,
umls-concept:C1547348,
umls-concept:C1552644,
umls-concept:C1704689,
umls-concept:C1705241,
umls-concept:C1823153,
umls-concept:C2349976,
umls-concept:C2911684
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pubmed:issue |
2
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pubmed:dateCreated |
2000-8-3
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pubmed:abstractText |
We recently reported that the number of gamma delta T cells was increased after infection with Escherichia coli in C3H/HeN mice. We here showed that an i.p. injection with native lipid A derived from E. coli induced an increase of gamma delta T cells in the peritoneal cavity of LPS-responsive C3H/HeN mice and, albeit to a lesser degree, also in LPS-hyporesponsive C3H/HeJ mice. The purified gamma delta T cells from C3H/HeN and C3H/HeJ mice expressed a canonical TCR repertoire encoded by V gamma 6-J gamma 1/V delta 1-D delta 2-J delta 2 gene segments and proliferated in response to the native lipid A derived from E. coli in a TCR-independent manner. The lipid A-reactive gamma delta T cells bearing canonical V gamma 6/V delta 1 expressed Toll-like receptor (TLR) 2 mRNA, while TLR4 mRNA was undetectable. Treatment with a TLR2 anti-sense oligonucleotide resulted in hyporesponsiveness of the gamma delta T cells to the native lipid A. TLR2-deficient mice showed an impaired increase of the gamma delta T cells following injection of native lipid A. These results suggest that TLR2 is involved in the activation of canonical V gamma 6/V delta 1 T cells by native E. coli lipid A.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid A,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
165
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
931-40
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10878368-Animals,
pubmed-meshheading:10878368-Antigens, CD14,
pubmed-meshheading:10878368-Ascitic Fluid,
pubmed-meshheading:10878368-Cell Line,
pubmed-meshheading:10878368-Cytokines,
pubmed-meshheading:10878368-Drosophila Proteins,
pubmed-meshheading:10878368-Escherichia coli Infections,
pubmed-meshheading:10878368-Female,
pubmed-meshheading:10878368-Gene Expression Regulation,
pubmed-meshheading:10878368-Genes, T-Cell Receptor delta,
pubmed-meshheading:10878368-Genes, T-Cell Receptor gamma,
pubmed-meshheading:10878368-Injections, Intraperitoneal,
pubmed-meshheading:10878368-Lipid A,
pubmed-meshheading:10878368-Lipopolysaccharides,
pubmed-meshheading:10878368-Lymphocyte Activation,
pubmed-meshheading:10878368-Membrane Glycoproteins,
pubmed-meshheading:10878368-Mice,
pubmed-meshheading:10878368-Mice, Inbred C3H,
pubmed-meshheading:10878368-Mice, Inbred C57BL,
pubmed-meshheading:10878368-Mice, Knockout,
pubmed-meshheading:10878368-Peritoneal Cavity,
pubmed-meshheading:10878368-RNA, Messenger,
pubmed-meshheading:10878368-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:10878368-Receptors, Cell Surface,
pubmed-meshheading:10878368-T-Lymphocyte Subsets,
pubmed-meshheading:10878368-Toll-Like Receptor 2,
pubmed-meshheading:10878368-Toll-Like Receptor 4,
pubmed-meshheading:10878368-Toll-Like Receptors
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pubmed:year |
2000
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pubmed:articleTitle |
Expression of toll-like receptor 2 on gamma delta T cells bearing invariant V gamma 6/V delta 1 induced by Escherichia coli infection in mice.
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pubmed:affiliation |
Laboratory of Host Defense and Germfree Life, Research Institute for Disease Mechanism and Control, and First Department of Surgery, Nagoya University School of Medicine, Nagoya, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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