Source:http://linkedlifedata.com/resource/pubmed/id/10878359
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2000-8-3
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| pubmed:abstractText |
The V kappa10 family of murine light chain Ig genes is composed of three members, two of which (V kappa 10A and V kappa 10B) are well used. V kappa 10C, the third member of this family, is not detected in any expressed Abs. Our previous work showed that V kappa 10C is structurally functional and can recombine, but mRNA levels in spleen were extremely low relative to those of V kappa 10A and V kappa 10B. Furthermore, while the V kappa 10C promoter was efficient in B cells, it was shown to work inefficiently in pre-B cell lines. Here, we extend our analysis of the V kappa 10 family and examine V kappa 10 gene accessibility, their representation in V kappa cDNA phage libraries, and the frequency and nature of rearrangements during different stages of B cell development. We demonstrate that V kappa 10C is under-represented in V kappa cDNA libraries, but that the frequency of its sterile transcripts in pre-B cells surpasses both V kappa 10A and V kappa 10B, indicating that the gene is as accessible as V kappa 10A and V kappa 10B to the recombination machinery. We also demonstrate that V kappa 10C recombines at a frequency equal to that of V kappa 10A in pre-B cells and has a normal nonproductive to productive recombination ratio. As B cells develop, however, both the frequency of V kappa 10C rearrangements and the presence of productive rearrangements decline, indicating that these cells are in some fashion being eliminated.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Jul
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
165
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
852-9
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| pubmed:dateRevised |
2005-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10878359-Animals,
pubmed-meshheading:10878359-B-Lymphocytes,
pubmed-meshheading:10878359-Bone Marrow Cells,
pubmed-meshheading:10878359-Cell Differentiation,
pubmed-meshheading:10878359-Cell Separation,
pubmed-meshheading:10878359-Female,
pubmed-meshheading:10878359-Gene Rearrangement, B-Lymphocyte,
pubmed-meshheading:10878359-Genes, Immunoglobulin,
pubmed-meshheading:10878359-Immunoglobulin Variable Region,
pubmed-meshheading:10878359-Immunoglobulin kappa-Chains,
pubmed-meshheading:10878359-Male,
pubmed-meshheading:10878359-Mice,
pubmed-meshheading:10878359-Mice, Inbred BALB C,
pubmed-meshheading:10878359-Multigene Family,
pubmed-meshheading:10878359-Recombination, Genetic,
pubmed-meshheading:10878359-Spleen,
pubmed-meshheading:10878359-Transcription, Genetic
|
| pubmed:year |
2000
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| pubmed:articleTitle |
Underutilization of the V kappa 10C gene in the B cell repertoire is due to the loss of productive VJ rearrangements during B cell development.
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| pubmed:affiliation |
Division of Monoclonal Antibodies and Division of Cellular and Gene Therapies, Food and Drug Administration, Center for Biologics Evaluation and Research, Rockville, MD 20852, USA. fitzsimmons@cber.fda.gov
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| pubmed:publicationType |
Journal Article
|